TY - JOUR
T1 - CD40 Ligand+ Microparticles From Human Atherosclerotic Plaques Stimulate Endothelial Proliferation and Angiogenesis. A Potential Mechanism for Intraplaque Neovascularization
AU - Leroyer, Aurélie S.
AU - Rautou, Pierre Emmanuel
AU - Silvestre, Jean Sébastien
AU - Castier, Yves
AU - Lesèche, Guy
AU - Devue, Cécile
AU - Duriez, Micheline
AU - Brandes, Ralf P.
AU - Lutgens, Esther
AU - Tedgui, Alain
AU - Boulanger, Chantal M.
N1 - Funding Information:
This work was supported by the Institut National de la Santé et de la Recherche Médicale, an educational grant from I.R.I. Servier (Suresnes, France), the Leducq Foundation (LINK project; to Dr. Tedgui), by ANR-05-COD-024, and by the European Vascular Genomics Network of Excellence (LSHM-CT-2003-503254; Brussels, Belgium). Dr. Leroyer was supported by the “Ministère de la Recherche et de l'Enseignement Supérieur,” Dr. Rautou by the “Direction Régionale des Affaires Sanitaires et Sociales d'Ile-de-France” (DRASSIF), and Dr. Boulanger by a “Contrat d'Interface Assistance Publique Hopitaux de Paris.”
PY - 2008/10/14
Y1 - 2008/10/14
N2 - Objectives: Our goal was to demonstrate that microparticles (MPs) are the endogenous signal leading to neovessel formation through CD40 ligation in human atherosclerotic plaques. Background: Vulnerable atherosclerotic plaques prone to rupture are characterized by an increased number of vasa vasorum and frequent intraplaque hemorrhage. Although inflammatory cytokines, growth factors, or CD40/CD40 ligand (CD40L) are possible candidates, the mechanism of atherosclerotic plaque neovascularization remains unknown. Atherosclerotic plaques contain large amounts of membrane-shed submicron MPs released after cell activation or apoptosis. Methods: Microparticles were isolated from endarterectomy specimens surgically obtained from 26 patients and characterized by phosphatidylserine exposure and specific markers of cellular origin. Results: Plaque MPs increased both endothelial proliferation assessed by 3H-thymidine incorporation and cell number and stimulated in vivo angiogenesis in Matrigel (BD Biosciences, San Diego, California) assays performed in wild-type and BalbC/Nude mice, whereas circulating MPs had no effect. Microparticles from symptomatic patients expressed more CD40L and were more potent in inducing endothelial proliferation, when compared with asymptomatic plaque MPs. Most of CD40L+ MPs (93%) isolated from human plaques were of macrophage origin. Microparticle-induced endothelial proliferation was impaired by CD40L or CD40-neutralizing antibodies and abolished after endothelial CD40-ribonucleic acid silencing. In addition, the proangiogenic effect of plaque MPs was abolished in Matrigel assays performed in the presence of CD40L-neutralizing antibodies or in CD40-deficient mice. Conclusions: These results demonstrate that MPs isolated from human atherosclerotic lesions express CD40L, stimulate endothelial cell proliferation after CD40 ligation, and promote in vivo angiogenesis. Therefore, MPs could represent a major determinant of intraplaque neovascularization and plaque vulnerability.
AB - Objectives: Our goal was to demonstrate that microparticles (MPs) are the endogenous signal leading to neovessel formation through CD40 ligation in human atherosclerotic plaques. Background: Vulnerable atherosclerotic plaques prone to rupture are characterized by an increased number of vasa vasorum and frequent intraplaque hemorrhage. Although inflammatory cytokines, growth factors, or CD40/CD40 ligand (CD40L) are possible candidates, the mechanism of atherosclerotic plaque neovascularization remains unknown. Atherosclerotic plaques contain large amounts of membrane-shed submicron MPs released after cell activation or apoptosis. Methods: Microparticles were isolated from endarterectomy specimens surgically obtained from 26 patients and characterized by phosphatidylserine exposure and specific markers of cellular origin. Results: Plaque MPs increased both endothelial proliferation assessed by 3H-thymidine incorporation and cell number and stimulated in vivo angiogenesis in Matrigel (BD Biosciences, San Diego, California) assays performed in wild-type and BalbC/Nude mice, whereas circulating MPs had no effect. Microparticles from symptomatic patients expressed more CD40L and were more potent in inducing endothelial proliferation, when compared with asymptomatic plaque MPs. Most of CD40L+ MPs (93%) isolated from human plaques were of macrophage origin. Microparticle-induced endothelial proliferation was impaired by CD40L or CD40-neutralizing antibodies and abolished after endothelial CD40-ribonucleic acid silencing. In addition, the proangiogenic effect of plaque MPs was abolished in Matrigel assays performed in the presence of CD40L-neutralizing antibodies or in CD40-deficient mice. Conclusions: These results demonstrate that MPs isolated from human atherosclerotic lesions express CD40L, stimulate endothelial cell proliferation after CD40 ligation, and promote in vivo angiogenesis. Therefore, MPs could represent a major determinant of intraplaque neovascularization and plaque vulnerability.
KW - VEGF
KW - angiogenesis
KW - atherosclerosis
KW - inflammation
KW - microparticles
KW - proliferation
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U2 - 10.1016/j.jacc.2008.07.032
DO - 10.1016/j.jacc.2008.07.032
M3 - Article
C2 - 18929241
AN - SCOPUS:53049091269
SN - 0735-1097
VL - 52
SP - 1302
EP - 1311
JO - Journal of the American College of Cardiology
JF - Journal of the American College of Cardiology
IS - 16
ER -