Abstract
The objective of this study was to investigate the contribution of the CD28 costimulatory molecules to allergen-induced primary and chronic inflammatory responses. To this end, we have developed and characterized a short ragweed allergen-induced asthma model involving sensitization of HLA-DQ transgenic mice followed by intranasal challenge with allergen. Forty-eight hours after primary challenge, sensitized DQ8 mice developed pulmonary eosinophilic inflammation, airway hyperreactivity, Th2 cytokines, and IgE/IgG1 Ab. This allergic inflammatory response was absent in H-2Aβ0 and DQ8/CD280 mice. Secondary rechallenge with allergen 4 weeks later induced even greater inflammatory changes in the airways of DQ8 mice with eosinophils being the predominant inflammatory cells while only pulmonary lymphocytosis was observed in DQ8/CD280 mice. No inflammation was detected in H-2Aβ0 mice. Proliferation and cytokine profile studies demonstrated that CD28 regulates T-cell activation and effector function. Therefore, CD28 is essential for the extrinsic asthma and can be a target for immunotherapy.
Original language | English (US) |
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Pages (from-to) | 83-94 |
Number of pages | 12 |
Journal | Clinical Immunology |
Volume | 106 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1 2003 |
Keywords
- Allergy
- Co-stimulation
- HLA
- Inflammation
- MHC
- Transgenic/knockout
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology