Ccne1 Overexpression Causes Chromosome Instability in Liver Cells and Liver Tumor Development in Mice

Khaled Aziz, Jazeel F. Limzerwala, Ines Sturmlechner, Erin Hurley, Cheng Zhang, Karthik B. Jeganathan, Grace Nelson, Steve Bronk, Raul O. Fierro Velasco, Erik Jan van Deursen, Daniel R. O'Brien, Jean-Pierre Kocher, Sameh A. Youssef, Janine H. van Ree, Alain de Bruin, Hilda van den Bos, Diana C.J. Spierings, Floris Foijer, Bart van de Sluis, Lewis Rowland RobertsGregory James Gores, Hu Li, Jan Van Deursen

Research output: Contribution to journalArticle

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Abstract

Background & Aims: The CCNE1 locus, which encodes cyclin E1, is amplified in many types of cancer cells and is activated in hepatocellular carcinomas (HCCs) from patients infected with hepatitis B virus or adeno-associated virus type 2, due to integration of the virus nearby. We investigated cell-cycle and oncogenic effects of cyclin E1 overexpression in tissues of mice. Methods: We generated mice with doxycycline-inducible expression of Ccne1 (Ccne1T mice) and activated overexpression of cyclin E1 from age 3 weeks onward. At 14 months of age, livers were collected from mice that overexpress cyclin E1 and nontransgenic mice (controls) and analyzed for tumor burden and by histology. Mouse embryonic fibroblasts (MEFs) and hepatocytes from Ccne1T and control mice were analyzed to determine the extent to which cyclin E1 overexpression perturbs S-phase entry, DNA replication, and numbers and structures of chromosomes. Tissues from 4-month-old Ccne1T and control mice (at that age were free of tumors) were analyzed for chromosome alterations, to investigate the mechanisms by which cyclin E1 predisposes hepatocytes to transformation. Results: Ccne1T mice developed more hepatocellular adenomas and HCCs than control mice. Tumors developed only in livers of Ccne1T mice, despite high levels of cyclin E1 in other tissues. Ccne1T MEFs had defects that promoted chromosome missegregation and aneuploidy, including incomplete replication of DNA, centrosome amplification, and formation of nonperpendicular mitotic spindles. Whereas Ccne1T mice accumulated near-diploid aneuploid cells in multiple tissues and organs, polyploidization was observed only in hepatocytes, with losses and gains of whole chromosomes, DNA damage, and oxidative stress. Conclusions: Livers, but not other tissues of mice with inducible overexpression of cyclin E1, develop tumors. More hepatocytes from the cyclin E1–overexpressing mice were polyploid than from control mice, and had losses or gains of whole chromosomes, DNA damage, and oxidative stress; all of these have been observed in human HCC cells. The increased risk of HCC in patients with hepatitis B virus or adeno-associated virus type 2 infection might involve activation of cyclin E1 and its effects on chromosomes and genomes of liver cells.

Original languageEnglish (US)
Pages (from-to)210-226.e12
JournalGastroenterology
Volume157
Issue number1
DOIs
StatePublished - Jul 1 2019

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Chromosomal Instability
Cyclins
Liver
Neoplasms
Chromosomes
Hepatocytes
Hepatocellular Carcinoma
Dependovirus
Aneuploidy
DNA Replication
Hepatitis B virus
DNA Damage
Oxidative Stress
Fibroblasts
Liver Cell Adenoma
Virus Integration
Chromosome Structures
Centrosome
Spindle Apparatus
Polyploidy

Keywords

  • AAV2
  • Chromosome Integrity
  • HBV
  • Hepatocarcinogenesis

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

Ccne1 Overexpression Causes Chromosome Instability in Liver Cells and Liver Tumor Development in Mice. / Aziz, Khaled; Limzerwala, Jazeel F.; Sturmlechner, Ines; Hurley, Erin; Zhang, Cheng; Jeganathan, Karthik B.; Nelson, Grace; Bronk, Steve; Fierro Velasco, Raul O.; van Deursen, Erik Jan; O'Brien, Daniel R.; Kocher, Jean-Pierre; Youssef, Sameh A.; van Ree, Janine H.; de Bruin, Alain; van den Bos, Hilda; Spierings, Diana C.J.; Foijer, Floris; van de Sluis, Bart; Roberts, Lewis Rowland; Gores, Gregory James; Li, Hu; Van Deursen, Jan.

In: Gastroenterology, Vol. 157, No. 1, 01.07.2019, p. 210-226.e12.

Research output: Contribution to journalArticle

Aziz, K, Limzerwala, JF, Sturmlechner, I, Hurley, E, Zhang, C, Jeganathan, KB, Nelson, G, Bronk, S, Fierro Velasco, RO, van Deursen, EJ, O'Brien, DR, Kocher, J-P, Youssef, SA, van Ree, JH, de Bruin, A, van den Bos, H, Spierings, DCJ, Foijer, F, van de Sluis, B, Roberts, LR, Gores, GJ, Li, H & Van Deursen, J 2019, 'Ccne1 Overexpression Causes Chromosome Instability in Liver Cells and Liver Tumor Development in Mice', Gastroenterology, vol. 157, no. 1, pp. 210-226.e12. https://doi.org/10.1053/j.gastro.2019.03.016
Aziz K, Limzerwala JF, Sturmlechner I, Hurley E, Zhang C, Jeganathan KB et al. Ccne1 Overexpression Causes Chromosome Instability in Liver Cells and Liver Tumor Development in Mice. Gastroenterology. 2019 Jul 1;157(1):210-226.e12. https://doi.org/10.1053/j.gastro.2019.03.016
Aziz, Khaled ; Limzerwala, Jazeel F. ; Sturmlechner, Ines ; Hurley, Erin ; Zhang, Cheng ; Jeganathan, Karthik B. ; Nelson, Grace ; Bronk, Steve ; Fierro Velasco, Raul O. ; van Deursen, Erik Jan ; O'Brien, Daniel R. ; Kocher, Jean-Pierre ; Youssef, Sameh A. ; van Ree, Janine H. ; de Bruin, Alain ; van den Bos, Hilda ; Spierings, Diana C.J. ; Foijer, Floris ; van de Sluis, Bart ; Roberts, Lewis Rowland ; Gores, Gregory James ; Li, Hu ; Van Deursen, Jan. / Ccne1 Overexpression Causes Chromosome Instability in Liver Cells and Liver Tumor Development in Mice. In: Gastroenterology. 2019 ; Vol. 157, No. 1. pp. 210-226.e12.
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abstract = "Background & Aims: The CCNE1 locus, which encodes cyclin E1, is amplified in many types of cancer cells and is activated in hepatocellular carcinomas (HCCs) from patients infected with hepatitis B virus or adeno-associated virus type 2, due to integration of the virus nearby. We investigated cell-cycle and oncogenic effects of cyclin E1 overexpression in tissues of mice. Methods: We generated mice with doxycycline-inducible expression of Ccne1 (Ccne1T mice) and activated overexpression of cyclin E1 from age 3 weeks onward. At 14 months of age, livers were collected from mice that overexpress cyclin E1 and nontransgenic mice (controls) and analyzed for tumor burden and by histology. Mouse embryonic fibroblasts (MEFs) and hepatocytes from Ccne1T and control mice were analyzed to determine the extent to which cyclin E1 overexpression perturbs S-phase entry, DNA replication, and numbers and structures of chromosomes. Tissues from 4-month-old Ccne1T and control mice (at that age were free of tumors) were analyzed for chromosome alterations, to investigate the mechanisms by which cyclin E1 predisposes hepatocytes to transformation. Results: Ccne1T mice developed more hepatocellular adenomas and HCCs than control mice. Tumors developed only in livers of Ccne1T mice, despite high levels of cyclin E1 in other tissues. Ccne1T MEFs had defects that promoted chromosome missegregation and aneuploidy, including incomplete replication of DNA, centrosome amplification, and formation of nonperpendicular mitotic spindles. Whereas Ccne1T mice accumulated near-diploid aneuploid cells in multiple tissues and organs, polyploidization was observed only in hepatocytes, with losses and gains of whole chromosomes, DNA damage, and oxidative stress. Conclusions: Livers, but not other tissues of mice with inducible overexpression of cyclin E1, develop tumors. More hepatocytes from the cyclin E1–overexpressing mice were polyploid than from control mice, and had losses or gains of whole chromosomes, DNA damage, and oxidative stress; all of these have been observed in human HCC cells. The increased risk of HCC in patients with hepatitis B virus or adeno-associated virus type 2 infection might involve activation of cyclin E1 and its effects on chromosomes and genomes of liver cells.",
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T1 - Ccne1 Overexpression Causes Chromosome Instability in Liver Cells and Liver Tumor Development in Mice

AU - Aziz, Khaled

AU - Limzerwala, Jazeel F.

AU - Sturmlechner, Ines

AU - Hurley, Erin

AU - Zhang, Cheng

AU - Jeganathan, Karthik B.

AU - Nelson, Grace

AU - Bronk, Steve

AU - Fierro Velasco, Raul O.

AU - van Deursen, Erik Jan

AU - O'Brien, Daniel R.

AU - Kocher, Jean-Pierre

AU - Youssef, Sameh A.

AU - van Ree, Janine H.

AU - de Bruin, Alain

AU - van den Bos, Hilda

AU - Spierings, Diana C.J.

AU - Foijer, Floris

AU - van de Sluis, Bart

AU - Roberts, Lewis Rowland

AU - Gores, Gregory James

AU - Li, Hu

AU - Van Deursen, Jan

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Background & Aims: The CCNE1 locus, which encodes cyclin E1, is amplified in many types of cancer cells and is activated in hepatocellular carcinomas (HCCs) from patients infected with hepatitis B virus or adeno-associated virus type 2, due to integration of the virus nearby. We investigated cell-cycle and oncogenic effects of cyclin E1 overexpression in tissues of mice. Methods: We generated mice with doxycycline-inducible expression of Ccne1 (Ccne1T mice) and activated overexpression of cyclin E1 from age 3 weeks onward. At 14 months of age, livers were collected from mice that overexpress cyclin E1 and nontransgenic mice (controls) and analyzed for tumor burden and by histology. Mouse embryonic fibroblasts (MEFs) and hepatocytes from Ccne1T and control mice were analyzed to determine the extent to which cyclin E1 overexpression perturbs S-phase entry, DNA replication, and numbers and structures of chromosomes. Tissues from 4-month-old Ccne1T and control mice (at that age were free of tumors) were analyzed for chromosome alterations, to investigate the mechanisms by which cyclin E1 predisposes hepatocytes to transformation. Results: Ccne1T mice developed more hepatocellular adenomas and HCCs than control mice. Tumors developed only in livers of Ccne1T mice, despite high levels of cyclin E1 in other tissues. Ccne1T MEFs had defects that promoted chromosome missegregation and aneuploidy, including incomplete replication of DNA, centrosome amplification, and formation of nonperpendicular mitotic spindles. Whereas Ccne1T mice accumulated near-diploid aneuploid cells in multiple tissues and organs, polyploidization was observed only in hepatocytes, with losses and gains of whole chromosomes, DNA damage, and oxidative stress. Conclusions: Livers, but not other tissues of mice with inducible overexpression of cyclin E1, develop tumors. More hepatocytes from the cyclin E1–overexpressing mice were polyploid than from control mice, and had losses or gains of whole chromosomes, DNA damage, and oxidative stress; all of these have been observed in human HCC cells. The increased risk of HCC in patients with hepatitis B virus or adeno-associated virus type 2 infection might involve activation of cyclin E1 and its effects on chromosomes and genomes of liver cells.

AB - Background & Aims: The CCNE1 locus, which encodes cyclin E1, is amplified in many types of cancer cells and is activated in hepatocellular carcinomas (HCCs) from patients infected with hepatitis B virus or adeno-associated virus type 2, due to integration of the virus nearby. We investigated cell-cycle and oncogenic effects of cyclin E1 overexpression in tissues of mice. Methods: We generated mice with doxycycline-inducible expression of Ccne1 (Ccne1T mice) and activated overexpression of cyclin E1 from age 3 weeks onward. At 14 months of age, livers were collected from mice that overexpress cyclin E1 and nontransgenic mice (controls) and analyzed for tumor burden and by histology. Mouse embryonic fibroblasts (MEFs) and hepatocytes from Ccne1T and control mice were analyzed to determine the extent to which cyclin E1 overexpression perturbs S-phase entry, DNA replication, and numbers and structures of chromosomes. Tissues from 4-month-old Ccne1T and control mice (at that age were free of tumors) were analyzed for chromosome alterations, to investigate the mechanisms by which cyclin E1 predisposes hepatocytes to transformation. Results: Ccne1T mice developed more hepatocellular adenomas and HCCs than control mice. Tumors developed only in livers of Ccne1T mice, despite high levels of cyclin E1 in other tissues. Ccne1T MEFs had defects that promoted chromosome missegregation and aneuploidy, including incomplete replication of DNA, centrosome amplification, and formation of nonperpendicular mitotic spindles. Whereas Ccne1T mice accumulated near-diploid aneuploid cells in multiple tissues and organs, polyploidization was observed only in hepatocytes, with losses and gains of whole chromosomes, DNA damage, and oxidative stress. Conclusions: Livers, but not other tissues of mice with inducible overexpression of cyclin E1, develop tumors. More hepatocytes from the cyclin E1–overexpressing mice were polyploid than from control mice, and had losses or gains of whole chromosomes, DNA damage, and oxidative stress; all of these have been observed in human HCC cells. The increased risk of HCC in patients with hepatitis B virus or adeno-associated virus type 2 infection might involve activation of cyclin E1 and its effects on chromosomes and genomes of liver cells.

KW - AAV2

KW - Chromosome Integrity

KW - HBV

KW - Hepatocarcinogenesis

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