Abstract
Background & Aims: The CCNE1 locus, which encodes cyclin E1, is amplified in many types of cancer cells and is activated in hepatocellular carcinomas (HCCs) from patients infected with hepatitis B virus or adeno-associated virus type 2, due to integration of the virus nearby. We investigated cell-cycle and oncogenic effects of cyclin E1 overexpression in tissues of mice. Methods: We generated mice with doxycycline-inducible expression of Ccne1 (Ccne1T mice) and activated overexpression of cyclin E1 from age 3 weeks onward. At 14 months of age, livers were collected from mice that overexpress cyclin E1 and nontransgenic mice (controls) and analyzed for tumor burden and by histology. Mouse embryonic fibroblasts (MEFs) and hepatocytes from Ccne1T and control mice were analyzed to determine the extent to which cyclin E1 overexpression perturbs S-phase entry, DNA replication, and numbers and structures of chromosomes. Tissues from 4-month-old Ccne1T and control mice (at that age were free of tumors) were analyzed for chromosome alterations, to investigate the mechanisms by which cyclin E1 predisposes hepatocytes to transformation. Results: Ccne1T mice developed more hepatocellular adenomas and HCCs than control mice. Tumors developed only in livers of Ccne1T mice, despite high levels of cyclin E1 in other tissues. Ccne1T MEFs had defects that promoted chromosome missegregation and aneuploidy, including incomplete replication of DNA, centrosome amplification, and formation of nonperpendicular mitotic spindles. Whereas Ccne1T mice accumulated near-diploid aneuploid cells in multiple tissues and organs, polyploidization was observed only in hepatocytes, with losses and gains of whole chromosomes, DNA damage, and oxidative stress. Conclusions: Livers, but not other tissues of mice with inducible overexpression of cyclin E1, develop tumors. More hepatocytes from the cyclin E1–overexpressing mice were polyploid than from control mice, and had losses or gains of whole chromosomes, DNA damage, and oxidative stress; all of these have been observed in human HCC cells. The increased risk of HCC in patients with hepatitis B virus or adeno-associated virus type 2 infection might involve activation of cyclin E1 and its effects on chromosomes and genomes of liver cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 210-226.e12 |
| Journal | Gastroenterology |
| Volume | 157 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1 2019 |
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Keywords
- AAV2
- Chromosome Integrity
- HBV
- Hepatocarcinogenesis
ASJC Scopus subject areas
- Hepatology
- Gastroenterology
Cite this
Ccne1 Overexpression Causes Chromosome Instability in Liver Cells and Liver Tumor Development in Mice. / Aziz, Khaled; Limzerwala, Jazeel F.; Sturmlechner, Ines; Hurley, Erin; Zhang, Cheng; Jeganathan, Karthik B.; Nelson, Grace; Bronk, Steve; Fierro Velasco, Raul O.; van Deursen, Erik Jan; O'Brien, Daniel R.; Kocher, Jean-Pierre; Youssef, Sameh A.; van Ree, Janine H.; de Bruin, Alain; van den Bos, Hilda; Spierings, Diana C.J.; Foijer, Floris; van de Sluis, Bart; Roberts, Lewis Rowland; Gores, Gregory James; Li, Hu; Van Deursen, Jan.
In: Gastroenterology, Vol. 157, No. 1, 01.07.2019, p. 210-226.e12.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ccne1 Overexpression Causes Chromosome Instability in Liver Cells and Liver Tumor Development in Mice
AU - Aziz, Khaled
AU - Limzerwala, Jazeel F.
AU - Sturmlechner, Ines
AU - Hurley, Erin
AU - Zhang, Cheng
AU - Jeganathan, Karthik B.
AU - Nelson, Grace
AU - Bronk, Steve
AU - Fierro Velasco, Raul O.
AU - van Deursen, Erik Jan
AU - O'Brien, Daniel R.
AU - Kocher, Jean-Pierre
AU - Youssef, Sameh A.
AU - van Ree, Janine H.
AU - de Bruin, Alain
AU - van den Bos, Hilda
AU - Spierings, Diana C.J.
AU - Foijer, Floris
AU - van de Sluis, Bart
AU - Roberts, Lewis Rowland
AU - Gores, Gregory James
AU - Li, Hu
AU - Van Deursen, Jan
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Background & Aims: The CCNE1 locus, which encodes cyclin E1, is amplified in many types of cancer cells and is activated in hepatocellular carcinomas (HCCs) from patients infected with hepatitis B virus or adeno-associated virus type 2, due to integration of the virus nearby. We investigated cell-cycle and oncogenic effects of cyclin E1 overexpression in tissues of mice. Methods: We generated mice with doxycycline-inducible expression of Ccne1 (Ccne1T mice) and activated overexpression of cyclin E1 from age 3 weeks onward. At 14 months of age, livers were collected from mice that overexpress cyclin E1 and nontransgenic mice (controls) and analyzed for tumor burden and by histology. Mouse embryonic fibroblasts (MEFs) and hepatocytes from Ccne1T and control mice were analyzed to determine the extent to which cyclin E1 overexpression perturbs S-phase entry, DNA replication, and numbers and structures of chromosomes. Tissues from 4-month-old Ccne1T and control mice (at that age were free of tumors) were analyzed for chromosome alterations, to investigate the mechanisms by which cyclin E1 predisposes hepatocytes to transformation. Results: Ccne1T mice developed more hepatocellular adenomas and HCCs than control mice. Tumors developed only in livers of Ccne1T mice, despite high levels of cyclin E1 in other tissues. Ccne1T MEFs had defects that promoted chromosome missegregation and aneuploidy, including incomplete replication of DNA, centrosome amplification, and formation of nonperpendicular mitotic spindles. Whereas Ccne1T mice accumulated near-diploid aneuploid cells in multiple tissues and organs, polyploidization was observed only in hepatocytes, with losses and gains of whole chromosomes, DNA damage, and oxidative stress. Conclusions: Livers, but not other tissues of mice with inducible overexpression of cyclin E1, develop tumors. More hepatocytes from the cyclin E1–overexpressing mice were polyploid than from control mice, and had losses or gains of whole chromosomes, DNA damage, and oxidative stress; all of these have been observed in human HCC cells. The increased risk of HCC in patients with hepatitis B virus or adeno-associated virus type 2 infection might involve activation of cyclin E1 and its effects on chromosomes and genomes of liver cells.
AB - Background & Aims: The CCNE1 locus, which encodes cyclin E1, is amplified in many types of cancer cells and is activated in hepatocellular carcinomas (HCCs) from patients infected with hepatitis B virus or adeno-associated virus type 2, due to integration of the virus nearby. We investigated cell-cycle and oncogenic effects of cyclin E1 overexpression in tissues of mice. Methods: We generated mice with doxycycline-inducible expression of Ccne1 (Ccne1T mice) and activated overexpression of cyclin E1 from age 3 weeks onward. At 14 months of age, livers were collected from mice that overexpress cyclin E1 and nontransgenic mice (controls) and analyzed for tumor burden and by histology. Mouse embryonic fibroblasts (MEFs) and hepatocytes from Ccne1T and control mice were analyzed to determine the extent to which cyclin E1 overexpression perturbs S-phase entry, DNA replication, and numbers and structures of chromosomes. Tissues from 4-month-old Ccne1T and control mice (at that age were free of tumors) were analyzed for chromosome alterations, to investigate the mechanisms by which cyclin E1 predisposes hepatocytes to transformation. Results: Ccne1T mice developed more hepatocellular adenomas and HCCs than control mice. Tumors developed only in livers of Ccne1T mice, despite high levels of cyclin E1 in other tissues. Ccne1T MEFs had defects that promoted chromosome missegregation and aneuploidy, including incomplete replication of DNA, centrosome amplification, and formation of nonperpendicular mitotic spindles. Whereas Ccne1T mice accumulated near-diploid aneuploid cells in multiple tissues and organs, polyploidization was observed only in hepatocytes, with losses and gains of whole chromosomes, DNA damage, and oxidative stress. Conclusions: Livers, but not other tissues of mice with inducible overexpression of cyclin E1, develop tumors. More hepatocytes from the cyclin E1–overexpressing mice were polyploid than from control mice, and had losses or gains of whole chromosomes, DNA damage, and oxidative stress; all of these have been observed in human HCC cells. The increased risk of HCC in patients with hepatitis B virus or adeno-associated virus type 2 infection might involve activation of cyclin E1 and its effects on chromosomes and genomes of liver cells.
KW - AAV2
KW - Chromosome Integrity
KW - HBV
KW - Hepatocarcinogenesis
UR - http://www.scopus.com/inward/record.url?scp=85065774406&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85065774406&partnerID=8YFLogxK
U2 - 10.1053/j.gastro.2019.03.016
DO - 10.1053/j.gastro.2019.03.016
M3 - Article
C2 - 30878468
AN - SCOPUS:85065774406
VL - 157
SP - 210-226.e12
JO - Gastroenterology
JF - Gastroenterology
SN - 0016-5085
IS - 1
ER -