TY - JOUR
T1 - Cats are protected against feline immunodeficiency virus infection following vaccination with a homologous AP-1 binding site-deleted mutant
AU - Kohmoto, M.
AU - Miyazawa, T.
AU - Sato, E.
AU - Uetsuka, K.
AU - Nishimura, Y.
AU - Ikeda, Y.
AU - Inada, G.
AU - Doi, K.
AU - Mikami, T.
PY - 1998
Y1 - 1998
N2 - Following establishment, via the vaginal route, of infection with an AP-1 binding-site deleted mutant (ΔAP-1) of feline immunodeficiency virus (FIV), cats were challenged with a homologous intact strain (TM2) of FIV. The cats were observed for 23 weeks to evaluate the efficacy of the ΔAP-1 against the homologous TM2 strain challenge. These two viruses were differentiated by Southern blotting after amplification of proviral DNA by semi-nested polymerase chain reaction in DNAs of peripheral blood mononuclear cells and tissues. A TM2-specific band was detected in one cat exposed to but not infected with ΔAP-1, but not in two ΔAP-1-infected. These results indicate that ΔAP-1 could protect against subsequent challenge with homologous FIV TM2 strain.
AB - Following establishment, via the vaginal route, of infection with an AP-1 binding-site deleted mutant (ΔAP-1) of feline immunodeficiency virus (FIV), cats were challenged with a homologous intact strain (TM2) of FIV. The cats were observed for 23 weeks to evaluate the efficacy of the ΔAP-1 against the homologous TM2 strain challenge. These two viruses were differentiated by Southern blotting after amplification of proviral DNA by semi-nested polymerase chain reaction in DNAs of peripheral blood mononuclear cells and tissues. A TM2-specific band was detected in one cat exposed to but not infected with ΔAP-1, but not in two ΔAP-1-infected. These results indicate that ΔAP-1 could protect against subsequent challenge with homologous FIV TM2 strain.
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U2 - 10.1007/s007050050422
DO - 10.1007/s007050050422
M3 - Article
C2 - 9787667
AN - SCOPUS:0031669466
SN - 0304-8608
VL - 143
SP - 1839
EP - 1845
JO - Archives of Virology
JF - Archives of Virology
IS - 9
ER -