Abstract
Purpose: Tissue engineered heart valves (TEHV) are being investigated to address the limitations of currently available valve prostheses. In order to advance a wide variety of TEHV approaches, the goal of this study was to develop a cardiac valve bioreactor system capable of conditioning living valves with a range of hydrodynamic conditions as well as capable of assessing hydrodynamic performance to ISO 5840 standards. Methods: A bioreactor system was designed based on the Windkessel approach. Novel features including a purpose-built valve chamber and pressure feedback control were incorporated to maintain asepsis while achieving a range of hydrodynamic conditions. The system was validated by testing hydrodynamic conditions with a bioprosthesis and by operating with cell culture medium for 4 weeks and living cells for 2 weeks. Results: The bioreactor system was able to produce a range of pressure and flow conditions from static to resting adult left ventricular outflow tract to pathological including hypertension. The system operated aseptically for 4 weeks and cell viability was maintained for 2 weeks. The system was also able to record the pressure and flow data needed to calculate effective orifice area and regurgitant fraction. Conclusions: We have developed a single bioreactor system that allows for step-wise conditioning protocols to be developed for each unique TEHV design as well as allows for hydrodynamic performance assessment.
Original language | English (US) |
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Pages (from-to) | 80-94 |
Number of pages | 15 |
Journal | Cardiovascular Engineering and Technology |
Volume | 10 |
Issue number | 1 |
DOIs | |
State | Published - Mar 15 2019 |
Keywords
- Biochemical stimulation
- Biomechanical stimulation
- ISO 5840
- Three-dimensional tissue culture
- Tissue engineered heart valve
ASJC Scopus subject areas
- Biomedical Engineering
- Cardiology and Cardiovascular Medicine