Candida albicans induces the release of inflammatory mediators from human peripheral blood monocytes

Mario Castro, Julie A. Bjoraker, Michael S. Rohrbach, Andrew Harold Limper

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Candida albicans (C. albicans) is a major nosocomial pathogen. We examined arachidonic acid (AA) and cytokine production by monocytes stimulated with C. albicans. [ 14C]-AA labeled monocytes released 8.9 ± 2.3% of the incorporated AA following stimulation with live C. albicans (C. albicans: monocyte of 16:1) (P = 0.0002). Prior studies indicate that soluble α-mannans and β-glucans antagonize mannose and β-glucan receptors, respectively. Preincubation of monocytes with α-mannan (100 μg/ml) caused 45.8 ± 5.7% inhibition of [ 14C]-AA release, whereas β-glucan (100 μg/ml) yielded 43.7 ± 6.0% inhibition (P < 0.05 for each compared to control). Additionally, monocytes stimulated with C. albicans also released interleukin-1β (IL-1β), tumor necrosis factor-α (TNFα), interleukin-6 (IL-6) and interleukin-8 (IL-8). However, α-mannan or β-glucan failed to inhibit IL-1β release. These data indicate that C. albicans induces monocytes to release AA and inflammatory cytokines. Furthermore, AA, but not cytokine liberation, is partially mediated by α-mannan and β-glucan components of the fungus.

Original languageEnglish (US)
Pages (from-to)107-122
Number of pages16
JournalInflammation
Volume20
Issue number1
DOIs
StatePublished - 1996

Fingerprint

Candida albicans
Glucans
Arachidonic Acid
Mannans
Monocytes
Cytokines
Interleukin-1
Interleukin-8
Interleukin-6
Fungi
Tumor Necrosis Factor-alpha

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology
  • Cell Biology

Cite this

Candida albicans induces the release of inflammatory mediators from human peripheral blood monocytes. / Castro, Mario; Bjoraker, Julie A.; Rohrbach, Michael S.; Limper, Andrew Harold.

In: Inflammation, Vol. 20, No. 1, 1996, p. 107-122.

Research output: Contribution to journalArticle

Castro, Mario ; Bjoraker, Julie A. ; Rohrbach, Michael S. ; Limper, Andrew Harold. / Candida albicans induces the release of inflammatory mediators from human peripheral blood monocytes. In: Inflammation. 1996 ; Vol. 20, No. 1. pp. 107-122.
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