Caloric Restriction and Rapamycin Differentially Alter Energy Metabolism in Yeast

Kyung Mi Choi, Seok Jin Hong, Jan Van Deursen, Sooah Kim, Kyoung Heon Kim, Cheol Koo Lee

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Rapamycin (RM), a drug that inhibits the mechanistic target of rapamycin (mTOR) pathway and responds to nutrient availability, seemingly mimics the effects of caloric restriction (CR) on healthy life span. However, the extent of the mechanistic overlap between RM and CR remains incompletely understood. Here, we compared the impact of CR and RM on cellular metabolic status. Both regimens maintained intracellular ATP through the chronological aging process and showed enhanced mitochondrial capacity. Comparative transcriptome analysis showed that CR had a stronger impact on global gene expression than RM. We observed a like impact on the metabolome and identified distinct metabolites affected by CR and RM. CR severely reduced the level of energy storage molecules including glycogen and lipid droplets, whereas RM did not. RM boosted the production of enzymes responsible for the breakdown of glycogen and lipid droplets. Collectively, these results provide insights into the distinct energy metabolism mechanisms induced by CR and RM, suggesting that these two anti-aging regimens might extend life span through distinctive pathways.

Original languageEnglish (US)
Pages (from-to)29-38
Number of pages10
JournalThe journals of gerontology. Series A, Biological sciences and medical sciences
Volume73
Issue number1
DOIs
StatePublished - Dec 12 2017
Externally publishedYes

Keywords

  • Metabolomics
  • Transcriptomics

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Fingerprint Dive into the research topics of 'Caloric Restriction and Rapamycin Differentially Alter Energy Metabolism in Yeast'. Together they form a unique fingerprint.

Cite this