Calcium-sequestering vesicles and contractile flagellar roots.

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The intracellular localization of calcium during contraction of striated flagellar roots of the green alga Tetraselmis subcordiformis was investigated using calcium pyroantimonate and calcium oxalate cytochemistry and energy-dispersive X-ray microanalysis. Vesicles (0.25 - 0.50 microns diameter) located in the anterior-most region of the cell are calcium-sequestering organelles. These vesicles exhibit a consistent and reproducible localization of electron-opaque pyroantimonate and oxalate salts of calcium. Striated contractile flagellar roots (CFR) show a periodic pattern of calcium antimonate (Ca-Sb) deposits following 'calcium shock' stimulation of contraction of this organelle. When CFRs are in a fully extended state they do not show Ca-Sb deposits. Deposits of Ca-Sb precipitate are present throughout the cytosol when CFRs are stimulated to contract. The implications of these findings with respect to the regulation of intracellular levels of 'free' Ca2+ and to the mechanism of CFR contraction are discussed.

Original languageEnglish (US)
Pages (from-to)433-443
Number of pages11
JournalJournal of Cell Science
Volume58
StatePublished - Dec 1982
Externally publishedYes

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Calcium
Calcium Oxalate
Organelles
Electron Probe Microanalysis
Histocytochemistry
Chlorophyta
Cytosol
Shock
Salts
Electrons
pyroantimonate
calcium antimonate

ASJC Scopus subject areas

  • Cell Biology

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Calcium-sequestering vesicles and contractile flagellar roots. / Salisbury, Jeffrey L.

In: Journal of Cell Science, Vol. 58, 12.1982, p. 433-443.

Research output: Contribution to journalArticle

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