TY - JOUR
T1 - Caffeine induces apoptosis in human neuroblastoma cell line SK-N-MC.
AU - Jang, Mi Hyeon
AU - Shin, Min Chul
AU - Kang, In Sug
AU - Baik, Hyung Hwan
AU - Cho, Yong Ho
AU - Chu, Jong Phill
AU - Kim, Ee Hwa
AU - Kim, Chang Ju
PY - 2002/10
Y1 - 2002/10
N2 - Caffeine is one of the most widely consumed neuroactive drugs, coming mostly from everyday beverages such as coffee and tea. To investigate whether caffeine induces apoptosis in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, flow cytometric analysis, DNA fragmentation assay, and caspase-3 enzyme assay were performed on SK-N-MC human neuroblastoma cells. Cells treated with caffeine at concentrations as high as 10 mM exhibited several characteristics of apoptosis. In addition, caffeine was shown to increase the caspase-3 activity. These results suggest that high-dose of caffeine induces apoptosis in human neuroblastoma cells, probably by increasing the caspase-3 enzyme activity.
AB - Caffeine is one of the most widely consumed neuroactive drugs, coming mostly from everyday beverages such as coffee and tea. To investigate whether caffeine induces apoptosis in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, flow cytometric analysis, DNA fragmentation assay, and caspase-3 enzyme assay were performed on SK-N-MC human neuroblastoma cells. Cells treated with caffeine at concentrations as high as 10 mM exhibited several characteristics of apoptosis. In addition, caffeine was shown to increase the caspase-3 activity. These results suggest that high-dose of caffeine induces apoptosis in human neuroblastoma cells, probably by increasing the caspase-3 enzyme activity.
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U2 - 10.3346/jkms.2002.17.5.674
DO - 10.3346/jkms.2002.17.5.674
M3 - Article
C2 - 12378022
AN - SCOPUS:0042263360
SN - 1011-8934
VL - 17
SP - 674
EP - 678
JO - Journal of Korean medical science
JF - Journal of Korean medical science
IS - 5
ER -