C-type natriuretic peptide has a negative inotropic effect on cardiac myocytes

Amiram Nir, Dong Fang Zhang, Ruhama Fixler, John C. Burnett, Yael Eilam, Yonathan Hasin

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

C-type natriuretic peptide (CNP) has vasodilatory and antimitogenic actions, but its role in the control of cardiac function is unclear. We studied the effect of CNP on cultured, beating neonatal rat cardiac myocytes. CNP caused a significant reduction in the amplitude of contraction and a significant accumulation of intracellular cyclic GMP. The effect of a membrane permeable cyclic GMP on cell contraction was similar to that of CNP. CNP caused no change in Ca2+ transients. Blockade of natriuretic peptide receptors abolished the effects of CNP on contraction and accumulation of intracellular cyclic GMP. Blockade of cyclic GMP-dependent protein kinase abolished the effect of CNP on myocyte contraction. We conclude that CNP has a negative inotropic effect on neonatal rat cardiac myocytes. The effect of CNP is mediated via natriuretic peptide receptor(s) causing elevation of intracellular cyclic GMP which possibly activates protein kinase and causes attenuation of myofilament sensitivity to Ca2+.

Original languageEnglish (US)
Pages (from-to)195-201
Number of pages7
JournalEuropean Journal of Pharmacology
Volume412
Issue number3
DOIs
StatePublished - Feb 2 2001

Keywords

  • Cell culture
  • Contractile function
  • Myocyte
  • Natriuretic peptide
  • Protein kinase

ASJC Scopus subject areas

  • Pharmacology

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