Brief report

Aldehyde dehydrogenase activity is a biomarker of primitive normal human mammary luminal cells

Peter Eirew, Nagarajan Kannan, David J H F Knapp, François Vaillant, Joanne T. Emerman, Geoffrey J. Lindeman, Jane E. Visvader, Connie J. Eaves

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Elevated aldehyde dehydrogenase (ALDH) expression/activity has been identified as an important biomarker of primitive cells in various normal and malignant human tissues. Here we examined the level and type of ALDH expression and activity in different subsets of phenotypically and functionally defined normal human mammary cells. We find that the most primitive human mammary stem and progenitor cell types with bilineage differentiation potential show low ALDH activity but undergo a marked, selective, and transient upregulation of ALDH activity at the point of commitment to the luminal lineage. This mirrors a corresponding change in transcripts and protein levels of ALDH1A3, an enzyme involved in retinoic acid synthesis and the most highly expressed ALDH gene in normal human mammary tissue. In contrast, ALDH1A1 is expressed at low levels in all mammary epithelial cells. These findings raise interesting questions about the reported association of ALDH activity with breast cancer stem cells and breast cancer prognosis.

Original languageEnglish (US)
Pages (from-to)344-348
Number of pages5
JournalStem Cells
Volume30
Issue number2
DOIs
StatePublished - Feb 1 2012
Externally publishedYes

Fingerprint

Aldehyde Dehydrogenase
Breast
Biomarkers
Stem Cells
Breast Neoplasms
Neoplastic Stem Cells
Tretinoin
Up-Regulation
Epithelial Cells
Enzymes
Genes
Proteins

Keywords

  • Breast cancer
  • Clonal assays
  • Progenitor cells
  • Tissue-specific stem cells

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Eirew, P., Kannan, N., Knapp, D. J. H. F., Vaillant, F., Emerman, J. T., Lindeman, G. J., ... Eaves, C. J. (2012). Brief report: Aldehyde dehydrogenase activity is a biomarker of primitive normal human mammary luminal cells. Stem Cells, 30(2), 344-348. https://doi.org/10.1002/stem.1001

Brief report : Aldehyde dehydrogenase activity is a biomarker of primitive normal human mammary luminal cells. / Eirew, Peter; Kannan, Nagarajan; Knapp, David J H F; Vaillant, François; Emerman, Joanne T.; Lindeman, Geoffrey J.; Visvader, Jane E.; Eaves, Connie J.

In: Stem Cells, Vol. 30, No. 2, 01.02.2012, p. 344-348.

Research output: Contribution to journalArticle

Eirew, P, Kannan, N, Knapp, DJHF, Vaillant, F, Emerman, JT, Lindeman, GJ, Visvader, JE & Eaves, CJ 2012, 'Brief report: Aldehyde dehydrogenase activity is a biomarker of primitive normal human mammary luminal cells', Stem Cells, vol. 30, no. 2, pp. 344-348. https://doi.org/10.1002/stem.1001
Eirew, Peter ; Kannan, Nagarajan ; Knapp, David J H F ; Vaillant, François ; Emerman, Joanne T. ; Lindeman, Geoffrey J. ; Visvader, Jane E. ; Eaves, Connie J. / Brief report : Aldehyde dehydrogenase activity is a biomarker of primitive normal human mammary luminal cells. In: Stem Cells. 2012 ; Vol. 30, No. 2. pp. 344-348.
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