Both DNA polymerases δ and ε contact active and stalled replication forks differently

Chuanhe Yu, Haiyun Gan, Zhiguo Zhang

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Three DNA polymerases, polymerases α, δ, and ε (Pol α, Pol δ, and Pol ε), are responsible for eukaryotic genome duplication. When DNA replication stress is encountered, DNA synthesis stalls until the stress is ameliorated. However, it is not known whether there is a difference in the association of each polymerase with active and stalled replication forks. Here, we show that each DNA polymerase has a distinct pattern of association with active and stalled replication forks. Pol α is enriched at extending Okazaki fragments of active and stalled forks. In contrast, although Pol δ contacts the nascent lagging strands of active and stalled forks, it binds to only the matured (and not elongating) Okazaki fragments of stalled forks. Pol ε has greater contact with the nascent single-stranded DNA (ssDNA) of the leading strand on active forks than on stalled forks. We propose that the configuration of DNA polymerases at stalled forks facilitates the resumption of DNA synthesis after stress removal.

Original languageEnglish (US)
Article numbere00190-17
JournalMolecular and cellular biology
Volume37
Issue number21
DOIs
StatePublished - Nov 1 2017

Keywords

  • ChIP-ssSeq
  • DNA polymerase
  • DNA replication
  • Replication stress
  • Strand-specific sequencing

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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