TY - JOUR
T1 - Biochemical and immunogenetic analysis of an immunodominant peptide (B6(dom1)) encoded by the classical H7 minor histocompatibility locus
AU - Eden, Peter A.
AU - Christianson, Gregory J.
AU - Fontaine, Pierre
AU - Wettstein, Peter J.
AU - Perreault, Claude
AU - Roopenian, Derry C.
PY - 1999/4/15
Y1 - 1999/4/15
N2 - Of the many minor histocompatibility (H) Ags that have been detected in mice, the ability to induce graft vs host disease (GVHD) after bone marrow transplantation is restricted to a limited number of immunodominant Ags. One such murine Ag, B6(dom1), is presented by the H2-Db MHC class I molecule. We present biochemical evidence that the natural B6(dom1) peptide is indistinguishable from AAPDNRETF, and we show that this peptide can be isolated from a wide array of tissues, with highest levels from the lymphoid organs and lung. Moreover, we employ a novel, somatic cell selection technique involving CTL-mediated immunoselection coupled with classical genetics, to show that B6(dom1) is encoded by the H7 minor H locus originally discovered ~40 years ago. These studies provide a molecular genetic framework for understanding B6(dom1), and exemplify the fact that mouse minor H loci that encode immunodominant CTL epitopes can correspond to classical H loci originally identified by their ability to confer strong resistance to tumor transplantation. Additionally, these studies demonstrate the utility of somatic cell selection approaches toward resolving HAg immunogenetics.
AB - Of the many minor histocompatibility (H) Ags that have been detected in mice, the ability to induce graft vs host disease (GVHD) after bone marrow transplantation is restricted to a limited number of immunodominant Ags. One such murine Ag, B6(dom1), is presented by the H2-Db MHC class I molecule. We present biochemical evidence that the natural B6(dom1) peptide is indistinguishable from AAPDNRETF, and we show that this peptide can be isolated from a wide array of tissues, with highest levels from the lymphoid organs and lung. Moreover, we employ a novel, somatic cell selection technique involving CTL-mediated immunoselection coupled with classical genetics, to show that B6(dom1) is encoded by the H7 minor H locus originally discovered ~40 years ago. These studies provide a molecular genetic framework for understanding B6(dom1), and exemplify the fact that mouse minor H loci that encode immunodominant CTL epitopes can correspond to classical H loci originally identified by their ability to confer strong resistance to tumor transplantation. Additionally, these studies demonstrate the utility of somatic cell selection approaches toward resolving HAg immunogenetics.
UR - http://www.scopus.com/inward/record.url?scp=0033561667&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033561667&partnerID=8YFLogxK
M3 - Article
C2 - 10201988
AN - SCOPUS:0033561667
SN - 0022-1767
VL - 162
SP - 4502
EP - 4510
JO - Journal of Immunology
JF - Journal of Immunology
IS - 8
ER -