Biochemical and immunogenetic analysis of an immunodominant peptide (B6(dom1)) encoded by the classical H7 minor histocompatibility locus

Peter A. Eden, Gregory J. Christianson, Pierre Fontaine, Peter J. Wettstein, Claude Perreault, Derry C. Roopenian

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Of the many minor histocompatibility (H) Ags that have been detected in mice, the ability to induce graft vs host disease (GVHD) after bone marrow transplantation is restricted to a limited number of immunodominant Ags. One such murine Ag, B6(dom1), is presented by the H2-Db MHC class I molecule. We present biochemical evidence that the natural B6(dom1) peptide is indistinguishable from AAPDNRETF, and we show that this peptide can be isolated from a wide array of tissues, with highest levels from the lymphoid organs and lung. Moreover, we employ a novel, somatic cell selection technique involving CTL-mediated immunoselection coupled with classical genetics, to show that B6(dom1) is encoded by the H7 minor H locus originally discovered ~40 years ago. These studies provide a molecular genetic framework for understanding B6(dom1), and exemplify the fact that mouse minor H loci that encode immunodominant CTL epitopes can correspond to classical H loci originally identified by their ability to confer strong resistance to tumor transplantation. Additionally, these studies demonstrate the utility of somatic cell selection approaches toward resolving HAg immunogenetics.

Original languageEnglish (US)
Pages (from-to)4502-4510
Number of pages9
JournalJournal of Immunology
Volume162
Issue number8
StatePublished - Apr 15 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Biochemical and immunogenetic analysis of an immunodominant peptide (B6(dom1)) encoded by the classical H7 minor histocompatibility locus'. Together they form a unique fingerprint.

Cite this