Abstract
The importance of lung macrophages in Pneumocystis-host interaction is well known, but little is known about the initial binding/colonization of the airway epithelium. Our prior studies have documented cell-signalling events that occur following binding of the organisms to lung epithelial cells; however, the receptors that mediate Pneumocystis attachment to lung surfaces have not yet been fully defined. Using affinity chromatography, we identified heat shock protein 5 (HSPA5), also known as GRP78, as a potential host receptor that may have relevance in Pneumocystis lung colonization. Pneumocystis carinii (Pc) organisms not only bound HSPA5 on a rat lung epithelial cell line, but also on primary rat airway epithelial cells (AECs). Furthermore, Pc bound CHO1 cells overexpressing HSPA5 more than the CHO1 parent line alone, supporting a role for Pc-HSPA5 protein interaction in mediating organism attachment. These results provide new insights into the interactions of Pneumocystis with host lung epithelium.
Original language | English (US) |
---|---|
Article number | 000864 |
Pages (from-to) | 1772-1777 |
Number of pages | 6 |
Journal | Journal of medical microbiology |
Volume | 67 |
Issue number | 12 |
DOIs | |
State | Published - Dec 2018 |
Keywords
- Pneumocystis Epithelium Adherence Receptor
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)