TY - JOUR
T1 - Autologous adipose-derived mesenchymal stem cells improve healing of coiled experimental saccular aneurysms
T2 - An angiographic and histopathological study
AU - Rouchaud, Aymeric
AU - Brinjikji, Waleed
AU - Dai, Daying
AU - Ding, Yong Hong
AU - Gunderson, Tina
AU - Schroeder, Dana
AU - Spelle, Laurent
AU - Kallmes, David F.
AU - Kadirvel, Ramanathan
N1 - Funding Information:
Funding This work was supported by research grant NS0767491 from the National Institute of Health and Medtronics. This work was partially funded by the SNIS Foundation Fellow Research Grant. AR was supported by research grants from the French Society of Radiology and Therese Planiol Foundation.
Funding Information:
This work was supported by research grant NS0767491 from the National Institute of Health and Medtronics. This work was partially funded by the SNIS Foundation Fellow Research Grant. AR was supported by research grants from the French Society of Radiology and Therese Planiol Foundation.
PY - 2018/1
Y1 - 2018/1
N2 - Purpose Long-term occlusion of coiled aneurysms frequently fails, probably because of poor intrasaccular healing and inadequate endothelialization across the aneurysm neck. The purpose of this study was to determine if attachment of autologous mesenchymal stem cells (MSCs) to platinum coils would improve the healing response in an elastase-induced aneurysm model in rabbits. Materials and methods With approval from the institutional animal care and use committee, aneurysms were created in rabbits and embolized with control platinum coils (Axium; Medtronic) (n=6) or coils seeded ex vivo with autologous adipose-tissue MSCs (n=7). Aneurysmal occlusion after embolization was evaluated at 1 month with angiography. Histological samples were analyzed by gross imaging and graded on the basis of neck and dome healing on H&E staining. Fibrosis was evaluated using a ratio of the total area presenting collagen. Endothelialization of the neck was quantitatively analyzed using CD31 immunohistochemistry. ?2 and Student’s t-test were used to compare groups. Results Healing score (11.5 vs 8.0, p=0.019), fibrosis ratio (10.3 vs 0.13, p=0.006) and endothelialization (902 262 µm2 vs 31 810 µm2, p=0.041) were significantly greater in the MSC group. The MSC group showed marked cellular proliferation and thrombus organization, with a continuous membrane bridging the neck of the aneurysm. Angiographic stable or progressive occlusion rate was significantly lower in the MSC group (0.00, 95% CI 0.00 to 0.41) compared with controls (0.67, 95% CI 0.22 to 0.96) (p=0.02). Conclusions Autologous MSCs attached to platinum coils significantly improve histological healing, as they result in improved neck endothelialization and collagen matrix formation within the aneurysm sac.
AB - Purpose Long-term occlusion of coiled aneurysms frequently fails, probably because of poor intrasaccular healing and inadequate endothelialization across the aneurysm neck. The purpose of this study was to determine if attachment of autologous mesenchymal stem cells (MSCs) to platinum coils would improve the healing response in an elastase-induced aneurysm model in rabbits. Materials and methods With approval from the institutional animal care and use committee, aneurysms were created in rabbits and embolized with control platinum coils (Axium; Medtronic) (n=6) or coils seeded ex vivo with autologous adipose-tissue MSCs (n=7). Aneurysmal occlusion after embolization was evaluated at 1 month with angiography. Histological samples were analyzed by gross imaging and graded on the basis of neck and dome healing on H&E staining. Fibrosis was evaluated using a ratio of the total area presenting collagen. Endothelialization of the neck was quantitatively analyzed using CD31 immunohistochemistry. ?2 and Student’s t-test were used to compare groups. Results Healing score (11.5 vs 8.0, p=0.019), fibrosis ratio (10.3 vs 0.13, p=0.006) and endothelialization (902 262 µm2 vs 31 810 µm2, p=0.041) were significantly greater in the MSC group. The MSC group showed marked cellular proliferation and thrombus organization, with a continuous membrane bridging the neck of the aneurysm. Angiographic stable or progressive occlusion rate was significantly lower in the MSC group (0.00, 95% CI 0.00 to 0.41) compared with controls (0.67, 95% CI 0.22 to 0.96) (p=0.02). Conclusions Autologous MSCs attached to platinum coils significantly improve histological healing, as they result in improved neck endothelialization and collagen matrix formation within the aneurysm sac.
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U2 - 10.1136/neurintsurg-2016-012867
DO - 10.1136/neurintsurg-2016-012867
M3 - Article
C2 - 28077523
AN - SCOPUS:85041720557
SN - 1759-8478
VL - 10
SP - 60
EP - 65
JO - Journal of neurointerventional surgery
JF - Journal of neurointerventional surgery
IS - 1
ER -