Autologous adipose-derived mesenchymal stem cells improve healing of coiled experimental saccular aneurysms: An angiographic and histopathological study

Aymeric Rouchaud, Waleed Brinjikji, Daying Dai, Yong Hong Ding, Tina Gunderson, Dana Schroeder, Laurent Spelle, David F Kallmes, Ramanathan D Kadirvel

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose Long-term occlusion of coiled aneurysms frequently fails, probably because of poor intrasaccular healing and inadequate endothelialization across the aneurysm neck. The purpose of this study was to determine if attachment of autologous mesenchymal stem cells (MSCs) to platinum coils would improve the healing response in an elastase-induced aneurysm model in rabbits. Materials and methods With approval from the institutional animal care and use committee, aneurysms were created in rabbits and embolized with control platinum coils (Axium; Medtronic) (n=6) or coils seeded ex vivo with autologous adipose-tissue MSCs (n=7). Aneurysmal occlusion after embolization was evaluated at 1 month with angiography. Histological samples were analyzed by gross imaging and graded on the basis of neck and dome healing on H&E staining. Fibrosis was evaluated using a ratio of the total area presenting collagen. Endothelialization of the neck was quantitatively analyzed using CD31 immunohistochemistry. ?2 and Student’s t-test were used to compare groups. Results Healing score (11.5 vs 8.0, p=0.019), fibrosis ratio (10.3 vs 0.13, p=0.006) and endothelialization (902 262 µm2 vs 31 810 µm2, p=0.041) were significantly greater in the MSC group. The MSC group showed marked cellular proliferation and thrombus organization, with a continuous membrane bridging the neck of the aneurysm. Angiographic stable or progressive occlusion rate was significantly lower in the MSC group (0.00, 95% CI 0.00 to 0.41) compared with controls (0.67, 95% CI 0.22 to 0.96) (p=0.02). Conclusions Autologous MSCs attached to platinum coils significantly improve histological healing, as they result in improved neck endothelialization and collagen matrix formation within the aneurysm sac.

Original languageEnglish (US)
Pages (from-to)60-65
Number of pages6
JournalJournal of NeuroInterventional Surgery
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2018

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Mesenchymal Stromal Cells
Aneurysm
Platinum
Neck
Fibrosis
Collagen
Animal Care Committees
Rabbits
Pancreatic Elastase
Adipose Tissue
Angiography
Thrombosis
Immunohistochemistry
Cell Proliferation
Organizations
Staining and Labeling
Students
Membranes

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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Autologous adipose-derived mesenchymal stem cells improve healing of coiled experimental saccular aneurysms : An angiographic and histopathological study. / Rouchaud, Aymeric; Brinjikji, Waleed; Dai, Daying; Ding, Yong Hong; Gunderson, Tina; Schroeder, Dana; Spelle, Laurent; Kallmes, David F; Kadirvel, Ramanathan D.

In: Journal of NeuroInterventional Surgery, Vol. 10, No. 1, 01.01.2018, p. 60-65.

Research output: Contribution to journalArticle

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abstract = "Purpose Long-term occlusion of coiled aneurysms frequently fails, probably because of poor intrasaccular healing and inadequate endothelialization across the aneurysm neck. The purpose of this study was to determine if attachment of autologous mesenchymal stem cells (MSCs) to platinum coils would improve the healing response in an elastase-induced aneurysm model in rabbits. Materials and methods With approval from the institutional animal care and use committee, aneurysms were created in rabbits and embolized with control platinum coils (Axium; Medtronic) (n=6) or coils seeded ex vivo with autologous adipose-tissue MSCs (n=7). Aneurysmal occlusion after embolization was evaluated at 1 month with angiography. Histological samples were analyzed by gross imaging and graded on the basis of neck and dome healing on H&E staining. Fibrosis was evaluated using a ratio of the total area presenting collagen. Endothelialization of the neck was quantitatively analyzed using CD31 immunohistochemistry. ?2 and Student’s t-test were used to compare groups. Results Healing score (11.5 vs 8.0, p=0.019), fibrosis ratio (10.3 vs 0.13, p=0.006) and endothelialization (902 262 µm2 vs 31 810 µm2, p=0.041) were significantly greater in the MSC group. The MSC group showed marked cellular proliferation and thrombus organization, with a continuous membrane bridging the neck of the aneurysm. Angiographic stable or progressive occlusion rate was significantly lower in the MSC group (0.00, 95{\%} CI 0.00 to 0.41) compared with controls (0.67, 95{\%} CI 0.22 to 0.96) (p=0.02). Conclusions Autologous MSCs attached to platinum coils significantly improve histological healing, as they result in improved neck endothelialization and collagen matrix formation within the aneurysm sac.",
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AU - Rouchaud, Aymeric

AU - Brinjikji, Waleed

AU - Dai, Daying

AU - Ding, Yong Hong

AU - Gunderson, Tina

AU - Schroeder, Dana

AU - Spelle, Laurent

AU - Kallmes, David F

AU - Kadirvel, Ramanathan D

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N2 - Purpose Long-term occlusion of coiled aneurysms frequently fails, probably because of poor intrasaccular healing and inadequate endothelialization across the aneurysm neck. The purpose of this study was to determine if attachment of autologous mesenchymal stem cells (MSCs) to platinum coils would improve the healing response in an elastase-induced aneurysm model in rabbits. Materials and methods With approval from the institutional animal care and use committee, aneurysms were created in rabbits and embolized with control platinum coils (Axium; Medtronic) (n=6) or coils seeded ex vivo with autologous adipose-tissue MSCs (n=7). Aneurysmal occlusion after embolization was evaluated at 1 month with angiography. Histological samples were analyzed by gross imaging and graded on the basis of neck and dome healing on H&E staining. Fibrosis was evaluated using a ratio of the total area presenting collagen. Endothelialization of the neck was quantitatively analyzed using CD31 immunohistochemistry. ?2 and Student’s t-test were used to compare groups. Results Healing score (11.5 vs 8.0, p=0.019), fibrosis ratio (10.3 vs 0.13, p=0.006) and endothelialization (902 262 µm2 vs 31 810 µm2, p=0.041) were significantly greater in the MSC group. The MSC group showed marked cellular proliferation and thrombus organization, with a continuous membrane bridging the neck of the aneurysm. Angiographic stable or progressive occlusion rate was significantly lower in the MSC group (0.00, 95% CI 0.00 to 0.41) compared with controls (0.67, 95% CI 0.22 to 0.96) (p=0.02). Conclusions Autologous MSCs attached to platinum coils significantly improve histological healing, as they result in improved neck endothelialization and collagen matrix formation within the aneurysm sac.

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