Autoimmune paraneoplastic cerebellar degeneration

Ultrastructural localization of antibody-binding sites in Purkinje cells

M. Rodriguez, Brian Patrick O'Neill, Vanda A Lennon, V. A. Lennon

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Sera from three of four patients with paraneoplastic cerebellar degeneration (PCD) associated with gynecologic cancer had antibodies that stained the cytoplasm of Purkinje cells in a characteristic discrete and coarsely granular pattern. No such antibodies were found in PCD patients with small cell cancer of the lung, in patients with cerebellar degeneration without cancer, in nonneurologic patients with small cell carcinoma or gynecologic cancer, or in normal subjects. Immunoelectron microscopy revealed that the antibodies of PCD bound to clusters of ribosomes, granular endoplasmic reticulum, and the trans-face of the vesicles of the Golgi complex in Purkinje cells. Immunostaining was localized in orderly arrays of stacked parallel cisternae of the endoplasmic reticulum in the perikaryon and dendritic processes. This pattern suggested that at least one autoantigen of PCD may be a glycoprotein specific for cerebellar tissue that is associated with the endoplasmic reticulum. Some patches of Purkinje plasma membrane also were immunostained.

Original languageEnglish (US)
Pages (from-to)1380-1386
Number of pages7
JournalNeurology
Volume38
Issue number9
StatePublished - 1988

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Paraneoplastic Cerebellar Degeneration
Antibody Binding Sites
Purkinje Cells
Endoplasmic Reticulum
Antibodies
Neoplasms
Small Cell Carcinoma
Immunoelectron Microscopy
Rough Endoplasmic Reticulum
Small Cell Lung Carcinoma
Autoantigens
Golgi Apparatus
Ribosomes
Glycoproteins
Cytoplasm
Cell Membrane
Serum

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Autoimmune paraneoplastic cerebellar degeneration : Ultrastructural localization of antibody-binding sites in Purkinje cells. / Rodriguez, M.; O'Neill, Brian Patrick; Lennon, Vanda A; Lennon, V. A.

In: Neurology, Vol. 38, No. 9, 1988, p. 1380-1386.

Research output: Contribution to journalArticle

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