Auto-delayed-type hypersensitivity induced in immunodeficient mice with modified self-antigens. V. Cellular autoreactivity directed against self-H-2D(d) subregion mediates the inflammatory responses

N. Tarcic, C. S. David, D. Naor

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

X-irradiated (250 rads) A mice injected with syngeneic trinitropohenylated spleen cells (Syn-TNP-SC) developed syngeneic delayed-type hypersensitivity (Syn-DTH) after footpad challenge with syngeneic concanavalin A-induced lymphoblasts (Syn-Con A blasts), as indicated by footpad swelling and lymph-node proliferation assays. Genetic analysis with different recombinant strains of mouse revealed that the H-2D(d) subregion restricts these immunological activities. A small immunogeneic entity was isolated from Syn-TNP-SC extract by gel filtration and affinity chromatography with anti-D(d) antibody. X-irradiated A mice injected with this antigen also generated inflammatory responses after challenge with Syn-Con A blasts. Analysis of the data obtained from the gel filtration and the affinity chromatography suggest that the Syn-DTH-stimulating antigen is a fragment of the H-2D(d) heavy chain with a molecular weight (MW) of 5,000 to 10,000. This fragment does not contain TNP molecules or sugar moieties and neither does it express affinity for antibodies against class II products. L(tk-) cells transfected with the H-2D(d) gene also induced Syn-DTH in X-irradiated A mice, while the parent cells failed to stimulate such a response. Taken together these findings indicate that the selection process in the thymus is not absolute and T cells recognizing self-major histocompatibility complex (MHC) products do migrate from this organ to the periphery of the immune system. The possible biological significance of these autoreactive cells is discussed.

Original languageEnglish (US)
Pages (from-to)184-190
Number of pages7
JournalImmunology
Volume67
Issue number2
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Auto-delayed-type hypersensitivity induced in immunodeficient mice with modified self-antigens. V. Cellular autoreactivity directed against self-H-2D(d) subregion mediates the inflammatory responses'. Together they form a unique fingerprint.

Cite this