ATP consumption rate per cross bridge depends on myosin heavy chain isoform

Young Soo Han, Paige C. Geiger, Mark J. Cody, Rebecca L. Macken, Gary C Sieck

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

In the present study, we tested the hypothesis that intrinsic differences in ATP consumption rate per cross bridge exist across rat diaphragm muscle (Diam) fibers expressing different myosin heavy chain (MHC) isoforms. During maximum Ca2+ activation (pCa 4.0) of single, Triton X-permeabilized Diam fibers, isometric ATP consumption rate was determined by using an NADH-linked fluorometric technique. The MHC concentration in single Diam fibers was determined by densitometric analysis of SDS-PAGE gels and comparison to a standard curve of known MHC concentrations. Isometric ATP consumption rate varied across Diam fibers expressing different MHC isoforms, being highest in fibers expressing MHC2X (1.14 ± 0.08 nmol·mm-3·s-1) and/or MHC2B (1.33± 0.08 nmol·mm-3·s-1), followed by fibers expressing MHC2A (0.77 ± 0.11 nmol·mm-3·s-1 and MHCSlow (0.46 ± 0.03 nmol·mm-3·s-1). These differences in ATP consumption rate also persisted when it was normalized for MHC concentration in single Diam fibers. Normalized ATP consumption rate for MHC concentration varied across Diam fibers expressing different MHC isoforms, being highest in fibers expressing MHC2X (2.02 ± 0.19 s-1) and/or MHC2B (2.64 ± 0.15 s-1), followed by fibers expressing MHC2A (1.57 ± 0.16 s-1) and MHCSlow (0.77 ± 0.05 s-1). On the basis of these results, we conclude that there are intrinsic differences in ATP consumption rate per cross bridge in Diam fibers expressing MHC isoforms.

Original languageEnglish (US)
Pages (from-to)2188-2196
Number of pages9
JournalJournal of Applied Physiology
Volume94
Issue number6
StatePublished - Jun 1 2003

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Myosin Heavy Chains
Diaphragm
Protein Isoforms
Adenosine Triphosphate
Muscles
NAD
Polyacrylamide Gel Electrophoresis
Gels

Keywords

  • Energetics
  • Skeletal muscle fibers
  • Tension cost

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Han, Y. S., Geiger, P. C., Cody, M. J., Macken, R. L., & Sieck, G. C. (2003). ATP consumption rate per cross bridge depends on myosin heavy chain isoform. Journal of Applied Physiology, 94(6), 2188-2196.

ATP consumption rate per cross bridge depends on myosin heavy chain isoform. / Han, Young Soo; Geiger, Paige C.; Cody, Mark J.; Macken, Rebecca L.; Sieck, Gary C.

In: Journal of Applied Physiology, Vol. 94, No. 6, 01.06.2003, p. 2188-2196.

Research output: Contribution to journalArticle

Han, YS, Geiger, PC, Cody, MJ, Macken, RL & Sieck, GC 2003, 'ATP consumption rate per cross bridge depends on myosin heavy chain isoform', Journal of Applied Physiology, vol. 94, no. 6, pp. 2188-2196.
Han YS, Geiger PC, Cody MJ, Macken RL, Sieck GC. ATP consumption rate per cross bridge depends on myosin heavy chain isoform. Journal of Applied Physiology. 2003 Jun 1;94(6):2188-2196.
Han, Young Soo ; Geiger, Paige C. ; Cody, Mark J. ; Macken, Rebecca L. ; Sieck, Gary C. / ATP consumption rate per cross bridge depends on myosin heavy chain isoform. In: Journal of Applied Physiology. 2003 ; Vol. 94, No. 6. pp. 2188-2196.
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