ATP consumption rate per cross bridge depends on myosin heavy chain isoform

Young Soo Han, Paige C. Geiger, Mark J. Cody, Rebecca L. Macken, Gary C. Sieck

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

In the present study, we tested the hypothesis that intrinsic differences in ATP consumption rate per cross bridge exist across rat diaphragm muscle (Diam) fibers expressing different myosin heavy chain (MHC) isoforms. During maximum Ca2+ activation (pCa 4.0) of single, Triton X-permeabilized Diam fibers, isometric ATP consumption rate was determined by using an NADH-linked fluorometric technique. The MHC concentration in single Diam fibers was determined by densitometric analysis of SDS-PAGE gels and comparison to a standard curve of known MHC concentrations. Isometric ATP consumption rate varied across Diam fibers expressing different MHC isoforms, being highest in fibers expressing MHC2X (1.14 ± 0.08 nmol·mm-3·s-1) and/or MHC2B (1.33± 0.08 nmol·mm-3·s-1), followed by fibers expressing MHC2A (0.77 ± 0.11 nmol·mm-3·s-1 and MHCSlow (0.46 ± 0.03 nmol·mm-3·s-1). These differences in ATP consumption rate also persisted when it was normalized for MHC concentration in single Diam fibers. Normalized ATP consumption rate for MHC concentration varied across Diam fibers expressing different MHC isoforms, being highest in fibers expressing MHC2X (2.02 ± 0.19 s-1) and/or MHC2B (2.64 ± 0.15 s-1), followed by fibers expressing MHC2A (1.57 ± 0.16 s-1) and MHCSlow (0.77 ± 0.05 s-1). On the basis of these results, we conclude that there are intrinsic differences in ATP consumption rate per cross bridge in Diam fibers expressing MHC isoforms.

Original languageEnglish (US)
Pages (from-to)2188-2196
Number of pages9
JournalJournal of applied physiology
Volume94
Issue number6
DOIs
StatePublished - Jun 1 2003

Keywords

  • Energetics
  • Skeletal muscle fibers
  • Tension cost

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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