Atomic force microscopy analysis of cell volume regulation

Chiara Spagnoli, Arthur Beyder, Stephen Besch, Frederick Sachs

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Cells swell in response a hypoosmotic challenge. By converting osmotic pressure to hydrostatic pressure at the cell membrane via van't Hoff's law, and converting that to tension via Laplace's law one predicts that the cell membrane should stretch and become stiff. We tested this prediction using the atomic force microscopy. During osmotic swelling cells did not become stiff and generally became softer. This result contradicts the assumption of the cell membrane as the constraining element in osmotic stress but is consistent with the cytoskeleton acting as a cross-linked gel. Models of the cells' response to osmotic stress must include energy terms for three-dimensional stresses.

Original languageEnglish (US)
Article number031916
JournalPhysical Review E - Statistical, Nonlinear, and Soft Matter Physics
Volume78
Issue number3
DOIs
StatePublished - Sep 22 2008

Fingerprint

Atomic Force Microscopy
atomic force microscopy
Cell
cells
Osmotic Stress
Membrane
osmosis
trucks
hydrostatic pressure
swelling
gels
Cytoskeleton
Hydrostatic Pressure
Swelling
Stretch
predictions
Laplace
Predict
Three-dimensional
energy

ASJC Scopus subject areas

  • Statistical and Nonlinear Physics
  • Statistics and Probability
  • Condensed Matter Physics

Cite this

Atomic force microscopy analysis of cell volume regulation. / Spagnoli, Chiara; Beyder, Arthur; Besch, Stephen; Sachs, Frederick.

In: Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, Vol. 78, No. 3, 031916, 22.09.2008.

Research output: Contribution to journalArticle

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