Associations between measles vaccine immunity and single-nucleotide polymorphisms in cytokine and cytokine receptor genes

Neelam Dhiman, Inna G. Ovsyannikova, Julie M Cunningham, Robert A. Vierkant, Richard B Kennedy, V. Shane Pankratz, Gregory A. Poland, Robert M. Jacobson

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Background. Cytokines are key regulators of measles vaccine humoral and cellular immunity. Single-nucleotide polymorphisms (SNPs) that are associated with differences in cytokine levels should also influence measles vaccine-induced immunity. Methods. We genotyped 118 measles-mumps-rubella- vaccinated subjects for SNPs from 6 cytokine genes (interleukin [IL]-2, IL-4, IL-10, IL-12A, IL-12B, and interferon [IFN]-γ) and their receptors (IL-2RA, IL-2RB, IL-4RA, IL-10RA, IL-10RB, IL-12RB1, IL-12RB2, and IFN-γR). Associations of SNPs with measles-specific antibodies, lymphoproliferation, and secreted cytokines were determined using χ2 tests and analyses of covariance. Results. We found significant associations (P < .05) between SNPs in the IL-2, IL-10, and IL-12RB genes and measles vaccine-induced immunity. The IVS1-100G (rs2069762) and the Ex2-34G (rs2069763) SNPs within the IL-2 gene were associated with high antibody and high lymphoproliferative responses, whereas SNPs within the IL-10 and IL-12R genes were associated with low antibody and lymphoproliferative responses to measles. SNPs within the IL-4RA and IL-12B genes varied significantly (P < .05) across immune response measures. Significant associations were also found between SNPs and secreted cytokine levels. Conclusions. Specific SNPs in the cytokine and cytokine receptor genes are significantly associated with variations in measures of the immune response to measles vaccination. These results need to be further validated in a larger cohort.

Original languageEnglish (US)
Pages (from-to)21-29
Number of pages9
JournalJournal of Infectious Diseases
Volume195
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

Measles Vaccine
Cytokine Receptors
Interleukins
Single Nucleotide Polymorphism
Immunity
Cytokines
Genes
Measles
Interleukin-12 Subunit p40
Interleukin-10
Interleukin-2
Interleukin-12 Subunit p35
Interferon Receptors
Mumps
Rubella
Antibodies
Humoral Immunity
Cellular Immunity
Interleukin-4
Interferons

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Associations between measles vaccine immunity and single-nucleotide polymorphisms in cytokine and cytokine receptor genes. / Dhiman, Neelam; Ovsyannikova, Inna G.; Cunningham, Julie M; Vierkant, Robert A.; Kennedy, Richard B; Pankratz, V. Shane; Poland, Gregory A.; Jacobson, Robert M.

In: Journal of Infectious Diseases, Vol. 195, No. 1, 01.01.2007, p. 21-29.

Research output: Contribution to journalArticle

Dhiman, Neelam ; Ovsyannikova, Inna G. ; Cunningham, Julie M ; Vierkant, Robert A. ; Kennedy, Richard B ; Pankratz, V. Shane ; Poland, Gregory A. ; Jacobson, Robert M. / Associations between measles vaccine immunity and single-nucleotide polymorphisms in cytokine and cytokine receptor genes. In: Journal of Infectious Diseases. 2007 ; Vol. 195, No. 1. pp. 21-29.
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abstract = "Background. Cytokines are key regulators of measles vaccine humoral and cellular immunity. Single-nucleotide polymorphisms (SNPs) that are associated with differences in cytokine levels should also influence measles vaccine-induced immunity. Methods. We genotyped 118 measles-mumps-rubella- vaccinated subjects for SNPs from 6 cytokine genes (interleukin [IL]-2, IL-4, IL-10, IL-12A, IL-12B, and interferon [IFN]-γ) and their receptors (IL-2RA, IL-2RB, IL-4RA, IL-10RA, IL-10RB, IL-12RB1, IL-12RB2, and IFN-γR). Associations of SNPs with measles-specific antibodies, lymphoproliferation, and secreted cytokines were determined using χ2 tests and analyses of covariance. Results. We found significant associations (P < .05) between SNPs in the IL-2, IL-10, and IL-12RB genes and measles vaccine-induced immunity. The IVS1-100G (rs2069762) and the Ex2-34G (rs2069763) SNPs within the IL-2 gene were associated with high antibody and high lymphoproliferative responses, whereas SNPs within the IL-10 and IL-12R genes were associated with low antibody and lymphoproliferative responses to measles. SNPs within the IL-4RA and IL-12B genes varied significantly (P < .05) across immune response measures. Significant associations were also found between SNPs and secreted cytokine levels. Conclusions. Specific SNPs in the cytokine and cytokine receptor genes are significantly associated with variations in measures of the immune response to measles vaccination. These results need to be further validated in a larger cohort.",
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AU - Vierkant, Robert A.

AU - Kennedy, Richard B

AU - Pankratz, V. Shane

AU - Poland, Gregory A.

AU - Jacobson, Robert M.

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N2 - Background. Cytokines are key regulators of measles vaccine humoral and cellular immunity. Single-nucleotide polymorphisms (SNPs) that are associated with differences in cytokine levels should also influence measles vaccine-induced immunity. Methods. We genotyped 118 measles-mumps-rubella- vaccinated subjects for SNPs from 6 cytokine genes (interleukin [IL]-2, IL-4, IL-10, IL-12A, IL-12B, and interferon [IFN]-γ) and their receptors (IL-2RA, IL-2RB, IL-4RA, IL-10RA, IL-10RB, IL-12RB1, IL-12RB2, and IFN-γR). Associations of SNPs with measles-specific antibodies, lymphoproliferation, and secreted cytokines were determined using χ2 tests and analyses of covariance. Results. We found significant associations (P < .05) between SNPs in the IL-2, IL-10, and IL-12RB genes and measles vaccine-induced immunity. The IVS1-100G (rs2069762) and the Ex2-34G (rs2069763) SNPs within the IL-2 gene were associated with high antibody and high lymphoproliferative responses, whereas SNPs within the IL-10 and IL-12R genes were associated with low antibody and lymphoproliferative responses to measles. SNPs within the IL-4RA and IL-12B genes varied significantly (P < .05) across immune response measures. Significant associations were also found between SNPs and secreted cytokine levels. Conclusions. Specific SNPs in the cytokine and cytokine receptor genes are significantly associated with variations in measures of the immune response to measles vaccination. These results need to be further validated in a larger cohort.

AB - Background. Cytokines are key regulators of measles vaccine humoral and cellular immunity. Single-nucleotide polymorphisms (SNPs) that are associated with differences in cytokine levels should also influence measles vaccine-induced immunity. Methods. We genotyped 118 measles-mumps-rubella- vaccinated subjects for SNPs from 6 cytokine genes (interleukin [IL]-2, IL-4, IL-10, IL-12A, IL-12B, and interferon [IFN]-γ) and their receptors (IL-2RA, IL-2RB, IL-4RA, IL-10RA, IL-10RB, IL-12RB1, IL-12RB2, and IFN-γR). Associations of SNPs with measles-specific antibodies, lymphoproliferation, and secreted cytokines were determined using χ2 tests and analyses of covariance. Results. We found significant associations (P < .05) between SNPs in the IL-2, IL-10, and IL-12RB genes and measles vaccine-induced immunity. The IVS1-100G (rs2069762) and the Ex2-34G (rs2069763) SNPs within the IL-2 gene were associated with high antibody and high lymphoproliferative responses, whereas SNPs within the IL-10 and IL-12R genes were associated with low antibody and lymphoproliferative responses to measles. SNPs within the IL-4RA and IL-12B genes varied significantly (P < .05) across immune response measures. Significant associations were also found between SNPs and secreted cytokine levels. Conclusions. Specific SNPs in the cytokine and cytokine receptor genes are significantly associated with variations in measures of the immune response to measles vaccination. These results need to be further validated in a larger cohort.

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