Assessment of tumor heterogeneity, as evidenced by gene expression profiles, pathway activation, and gene copy number, in patients with multifocal invasive lobular breast tumors

Nadine Norton, Pooja P. Advani, Daniel J. Serie, Xochiquetzal J. Geiger, Brian M. Necela, Bianca C. Axenfeld, Jennifer M. Kachergus, Ryan W. Feathers, Jennifer M. Carr, Juliana Crook, Alvaro Moreno Aspitia, Panagiotis Z Anastasiadis, Edith A. Perez, E Aubrey Thompson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Invasive lobular carcinoma (ILC) comprises approximately ∼10-20% of breast cancers. In general, multifocal/multicentric (MF/MC) breast cancer has been associated with an increased rate of regional lymph node metastases. Tumor heterogeneity between foci represents a largely unstudied source of genomic variation in those rare patients with MF/MC ILC. Methods: We characterized gene expression and copy number in 2 ormore foci from 11 patients with MF/MC ILC (all ER+, HER2-) and adjacent normal tissue. RNA and DNA were extracted from 3x1.5mm cores from all foci. Gene expression (730 genes) and copy number (80 genes) were measured using Nanostring PanCancer and Cancer CNV panels. Linear mixed models were employed to compare expression in tumor versus normal samples fromthe same patient, and to assess heterogeneity (variability) in expression among multiple ILC within an individual. Results: 35 and 34 genes were upregulated (FC>2) and down-regulated (FC

Original languageEnglish (US)
Article numbere0153411
JournalPLoS One
Volume11
Issue number4
DOIs
StatePublished - Apr 1 2016

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Lobular Carcinoma
gene dosage
Gene Dosage
Transcriptome
Gene expression
breast neoplasms
carcinoma
Tumors
Genes
Chemical activation
Breast Neoplasms
gene expression
neoplasms
Neoplasms
Gene Expression
genes
metastasis
RNA
Tissue
lymph nodes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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Assessment of tumor heterogeneity, as evidenced by gene expression profiles, pathway activation, and gene copy number, in patients with multifocal invasive lobular breast tumors. / Norton, Nadine; Advani, Pooja P.; Serie, Daniel J.; Geiger, Xochiquetzal J.; Necela, Brian M.; Axenfeld, Bianca C.; Kachergus, Jennifer M.; Feathers, Ryan W.; Carr, Jennifer M.; Crook, Juliana; Moreno Aspitia, Alvaro; Anastasiadis, Panagiotis Z; Perez, Edith A.; Thompson, E Aubrey.

In: PLoS One, Vol. 11, No. 4, e0153411, 01.04.2016.

Research output: Contribution to journalArticle

Norton, Nadine ; Advani, Pooja P. ; Serie, Daniel J. ; Geiger, Xochiquetzal J. ; Necela, Brian M. ; Axenfeld, Bianca C. ; Kachergus, Jennifer M. ; Feathers, Ryan W. ; Carr, Jennifer M. ; Crook, Juliana ; Moreno Aspitia, Alvaro ; Anastasiadis, Panagiotis Z ; Perez, Edith A. ; Thompson, E Aubrey. / Assessment of tumor heterogeneity, as evidenced by gene expression profiles, pathway activation, and gene copy number, in patients with multifocal invasive lobular breast tumors. In: PLoS One. 2016 ; Vol. 11, No. 4.
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AU - Advani, Pooja P.

AU - Serie, Daniel J.

AU - Geiger, Xochiquetzal J.

AU - Necela, Brian M.

AU - Axenfeld, Bianca C.

AU - Kachergus, Jennifer M.

AU - Feathers, Ryan W.

AU - Carr, Jennifer M.

AU - Crook, Juliana

AU - Moreno Aspitia, Alvaro

AU - Anastasiadis, Panagiotis Z

AU - Perez, Edith A.

AU - Thompson, E Aubrey

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