Application of tissue microarray technology to the study of non-Hodgkin's and Hodgkin's lymphoma

Cyrus V. Hedvat, Abhijith Hegde, Raju S K Chaganti, Beiyun Chen, Jing Qin, Daniel A. Filippa, Stephen D. Nimer, Julie Teruya-Feldstein

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

The immunohistochemical analysis of lymphoid neoplasms has led to refined classification schemes based on the profile of antigen expression and correlation with morphological, cytogenetic, molecular, and clinical features. Tissue microarrays (TMAs) are a powerful tool to rapidly characterize the phenotypic profile of a large number of samples. We show that this technique can be readily applied to the study of lymphoma by examining the expression profile of a series of 193 B-cell non-Hodgkin's lymphomas (NHLs) and 29 Hodgkin's lymphomas (HLs) using immunohistochemistry and in situ hybridization (ISH). The NHL cases were studied for the expression of commonly used markers - including CD3, CD5, CD10, CD20, CD23, CD30, CD43, Bcl-2, and cyclin D1 by immunohistochemical staining of TMAs - and these results were compared with whole sections (WS) of the same cases. We found a high degree of correlation between the results achieved with TMAs or WS (86% to 100% of cases). P53 and MIB-1 staining were studied, and the results were similar to that reported in the literature. HL cases were stained for CD20, CD30, CD15 (LeuM1), and latent membrane protein 1 expression, and ISH was performed using probes for EBER-1 and-2 transcripts. The re suits from HL cases on TMA sections matched exactly with those of WS. We correlated cytogenetic results with immunohistochemical stains and morphology in cases of mantle cell lymphoma [t(11;14)(q13;q32)] and follicular lymphoma [t(14;18)(q32;q24)]. This extensive expression profile of B-cell NHLs and HL tissues discloses the ability of TMAs to rapidly screen a large series of cases and represents the first report of method validation for this technique in the study of lymphoma.

Original languageEnglish (US)
Pages (from-to)968-974
Number of pages7
JournalHuman Pathology
Volume33
Issue number10
DOIs
StatePublished - Oct 1 2002
Externally publishedYes

Fingerprint

Hodgkin Disease
Non-Hodgkin's Lymphoma
Technology
B-Cell Lymphoma
Cytogenetics
In Situ Hybridization
Lymphoma
Staining and Labeling
Mantle-Cell Lymphoma
Follicular Lymphoma
Cyclin D1
Membrane Proteins
Coloring Agents
Immunohistochemistry
Antigens
Neoplasms

Keywords

  • Hodgkin's
  • Lymphoma
  • Tissue microarray

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Hedvat, C. V., Hegde, A., Chaganti, R. S. K., Chen, B., Qin, J., Filippa, D. A., ... Teruya-Feldstein, J. (2002). Application of tissue microarray technology to the study of non-Hodgkin's and Hodgkin's lymphoma. Human Pathology, 33(10), 968-974. https://doi.org/10.1053/hupa.2002.127438

Application of tissue microarray technology to the study of non-Hodgkin's and Hodgkin's lymphoma. / Hedvat, Cyrus V.; Hegde, Abhijith; Chaganti, Raju S K; Chen, Beiyun; Qin, Jing; Filippa, Daniel A.; Nimer, Stephen D.; Teruya-Feldstein, Julie.

In: Human Pathology, Vol. 33, No. 10, 01.10.2002, p. 968-974.

Research output: Contribution to journalArticle

Hedvat, CV, Hegde, A, Chaganti, RSK, Chen, B, Qin, J, Filippa, DA, Nimer, SD & Teruya-Feldstein, J 2002, 'Application of tissue microarray technology to the study of non-Hodgkin's and Hodgkin's lymphoma', Human Pathology, vol. 33, no. 10, pp. 968-974. https://doi.org/10.1053/hupa.2002.127438
Hedvat, Cyrus V. ; Hegde, Abhijith ; Chaganti, Raju S K ; Chen, Beiyun ; Qin, Jing ; Filippa, Daniel A. ; Nimer, Stephen D. ; Teruya-Feldstein, Julie. / Application of tissue microarray technology to the study of non-Hodgkin's and Hodgkin's lymphoma. In: Human Pathology. 2002 ; Vol. 33, No. 10. pp. 968-974.
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