Apoptotic cell death of human interstitial cells of Cajal

S. J. Gibbons, R. De Giorgio, M. S. Faussone Pellegrini, M. M. Garrity-Park, S. M. Miller, P. F. Schmalz, T. M. Young-Fadok, David Larson, Eric Dozois, Michael Camilleri, V. Stanghellini, J. H. Szurszewski, Gianrico Farrugia

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Interstitial cells of Cajal (ICC) are specialized mesenchyme-derived cells that regulate contractility and excitability of many smooth muscles with loss of ICC seen in a variety of gut motility disorders. Maintenance of ICC numbers is tightly regulated, with several factors known to regulate proliferation. In contrast, the fate of ICC is not established. The aim of this study was to investigate whether apoptosis plays a role in the regulation of ICC numbers in the normal colon. ICC were identified by immunolabelling for the c-Kit receptor tyrosine kinase and by electron microscopy. Apoptosis was detected in colon tissue by immunolabelling for activated caspase-3, terminal dUTP nucleotide end labelling and by ultrastructural changes in the cells. Apoptotic ICC were identified and counted in double-labelled tissue sections. They were identified in all layers of the colonic muscle. In the muscularis propria 1.5 ± 0.2% of ICC were positive for activated caspase-3 and in the circular muscle layer 2.1 ± 0.9% of ICC were positive for TUNEL. Apoptotic ICC were identified by electron microscopy. Apoptotic cell death is a continuing process in ICC. The level of apoptosis in ICC in healthy colon indicates that these cells must be continually regenerated to maintain intact networks.

Original languageEnglish (US)
Pages (from-to)85-93
Number of pages9
JournalNeurogastroenterology and Motility
Volume21
Issue number1
DOIs
StatePublished - Jan 2009

Fingerprint

Interstitial Cells of Cajal
Cell Death
Colon
Apoptosis
Caspase 3
Electron Microscopy
Cell Count
Proto-Oncogene Proteins c-kit
Muscles
In Situ Nick-End Labeling
Mesoderm
Protein-Tyrosine Kinases
Smooth Muscle

Keywords

  • Caspases
  • Colon
  • Electron microscopy
  • Gastrointestinal motility
  • TUNEL

ASJC Scopus subject areas

  • Endocrine and Autonomic Systems
  • Gastroenterology
  • Physiology

Cite this

Gibbons, S. J., De Giorgio, R., Faussone Pellegrini, M. S., Garrity-Park, M. M., Miller, S. M., Schmalz, P. F., ... Farrugia, G. (2009). Apoptotic cell death of human interstitial cells of Cajal. Neurogastroenterology and Motility, 21(1), 85-93. https://doi.org/10.1111/j.1365-2982.2008.01185.x

Apoptotic cell death of human interstitial cells of Cajal. / Gibbons, S. J.; De Giorgio, R.; Faussone Pellegrini, M. S.; Garrity-Park, M. M.; Miller, S. M.; Schmalz, P. F.; Young-Fadok, T. M.; Larson, David; Dozois, Eric; Camilleri, Michael; Stanghellini, V.; Szurszewski, J. H.; Farrugia, Gianrico.

In: Neurogastroenterology and Motility, Vol. 21, No. 1, 01.2009, p. 85-93.

Research output: Contribution to journalArticle

Gibbons, SJ, De Giorgio, R, Faussone Pellegrini, MS, Garrity-Park, MM, Miller, SM, Schmalz, PF, Young-Fadok, TM, Larson, D, Dozois, E, Camilleri, M, Stanghellini, V, Szurszewski, JH & Farrugia, G 2009, 'Apoptotic cell death of human interstitial cells of Cajal', Neurogastroenterology and Motility, vol. 21, no. 1, pp. 85-93. https://doi.org/10.1111/j.1365-2982.2008.01185.x
Gibbons SJ, De Giorgio R, Faussone Pellegrini MS, Garrity-Park MM, Miller SM, Schmalz PF et al. Apoptotic cell death of human interstitial cells of Cajal. Neurogastroenterology and Motility. 2009 Jan;21(1):85-93. https://doi.org/10.1111/j.1365-2982.2008.01185.x
Gibbons, S. J. ; De Giorgio, R. ; Faussone Pellegrini, M. S. ; Garrity-Park, M. M. ; Miller, S. M. ; Schmalz, P. F. ; Young-Fadok, T. M. ; Larson, David ; Dozois, Eric ; Camilleri, Michael ; Stanghellini, V. ; Szurszewski, J. H. ; Farrugia, Gianrico. / Apoptotic cell death of human interstitial cells of Cajal. In: Neurogastroenterology and Motility. 2009 ; Vol. 21, No. 1. pp. 85-93.
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