Antiurolithic activity and biotransformation of galloylquinic acids by Aspergillus alliaceus ATCC10060, Aspergillus brasiliensis ATCC 16404, and Cunninghamella elegans ATCC 10028b

Mohamed Abd El-Salam, Niege Furtado, Zejfa Haskic, John C Lieske, Jairo Bastos

Research output: Contribution to journalArticle

Abstract

Copaifera lucens n-butanolic fraction (BF) was used as a source of galloylquinic acids, and aerobically incubated with Aspergillus alliaceus ATCC10060, Aspergillus brasiliensis ATCC 16404, and Cunninghamella elegans ATCC 10028b cultures for 60 and 120 h. Out of the three studied filamentous fungi, A. alliaceus ATCC10060 was able to degrade galloylquinic acids into one major metabolite, 3-O-methylgallic acid (M1). The product was identified by 1H NMR, UPLC-MS/MS, and its potential effect on calcium oxalate monohydrate (COM) crystal binding to Madin-Darby canine kidney cells type I surface was studied. Renal cells pretreatment with BF and M1 for 3 h significantly decreased COM crystaladherence at 50 μg/mL and 5 μM, respectively. Moreover, both M1 and BF significantly reduced the surface expression of COM-binding proteins annexin A1 (ANXA1) and heat shock protein 90 (HSP90), respectively as evidenced by Western blot analysis of membrane, cytosolic, and whole cell lysate fractions. The compounds also showed antioxidant activities in a DPPH assay.

Original languageEnglish (US)
Article number101012
JournalBiocatalysis and Agricultural Biotechnology
Volume18
DOIs
StatePublished - Mar 1 2019

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Aspergillus alliaceus
Cunninghamella elegans
Cunninghamella
Calcium Oxalate
calcium oxalate
Aspergillus
Biotransformation
biotransformation
Calcium
kidney cells
Acids
acids
Copaifera
HSP90 Heat-Shock Proteins
Annexins
Madin Darby Canine Kidney Cells
Metabolites
Antioxidants
Fungi
Fabaceae

Keywords

  • Biotransformation
  • Calcium oxalate monohydrate
  • Copaifera lucens
  • Filamentous fungi
  • Galloylquinic acids
  • Urolithiasis

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science

Cite this

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title = "Antiurolithic activity and biotransformation of galloylquinic acids by Aspergillus alliaceus ATCC10060, Aspergillus brasiliensis ATCC 16404, and Cunninghamella elegans ATCC 10028b",
abstract = "Copaifera lucens n-butanolic fraction (BF) was used as a source of galloylquinic acids, and aerobically incubated with Aspergillus alliaceus ATCC10060, Aspergillus brasiliensis ATCC 16404, and Cunninghamella elegans ATCC 10028b cultures for 60 and 120 h. Out of the three studied filamentous fungi, A. alliaceus ATCC10060 was able to degrade galloylquinic acids into one major metabolite, 3-O-methylgallic acid (M1). The product was identified by 1H NMR, UPLC-MS/MS, and its potential effect on calcium oxalate monohydrate (COM) crystal binding to Madin-Darby canine kidney cells type I surface was studied. Renal cells pretreatment with BF and M1 for 3 h significantly decreased COM crystaladherence at 50 μg/mL and 5 μM, respectively. Moreover, both M1 and BF significantly reduced the surface expression of COM-binding proteins annexin A1 (ANXA1) and heat shock protein 90 (HSP90), respectively as evidenced by Western blot analysis of membrane, cytosolic, and whole cell lysate fractions. The compounds also showed antioxidant activities in a DPPH assay.",
keywords = "Biotransformation, Calcium oxalate monohydrate, Copaifera lucens, Filamentous fungi, Galloylquinic acids, Urolithiasis",
author = "{Abd El-Salam}, Mohamed and Niege Furtado and Zejfa Haskic and Lieske, {John C} and Jairo Bastos",
year = "2019",
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doi = "10.1016/j.bcab.2019.01.050",
language = "English (US)",
volume = "18",
journal = "Biocatalysis and Agricultural Biotechnology",
issn = "1878-8181",
publisher = "Elsevier BV",

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T1 - Antiurolithic activity and biotransformation of galloylquinic acids by Aspergillus alliaceus ATCC10060, Aspergillus brasiliensis ATCC 16404, and Cunninghamella elegans ATCC 10028b

AU - Abd El-Salam, Mohamed

AU - Furtado, Niege

AU - Haskic, Zejfa

AU - Lieske, John C

AU - Bastos, Jairo

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Copaifera lucens n-butanolic fraction (BF) was used as a source of galloylquinic acids, and aerobically incubated with Aspergillus alliaceus ATCC10060, Aspergillus brasiliensis ATCC 16404, and Cunninghamella elegans ATCC 10028b cultures for 60 and 120 h. Out of the three studied filamentous fungi, A. alliaceus ATCC10060 was able to degrade galloylquinic acids into one major metabolite, 3-O-methylgallic acid (M1). The product was identified by 1H NMR, UPLC-MS/MS, and its potential effect on calcium oxalate monohydrate (COM) crystal binding to Madin-Darby canine kidney cells type I surface was studied. Renal cells pretreatment with BF and M1 for 3 h significantly decreased COM crystaladherence at 50 μg/mL and 5 μM, respectively. Moreover, both M1 and BF significantly reduced the surface expression of COM-binding proteins annexin A1 (ANXA1) and heat shock protein 90 (HSP90), respectively as evidenced by Western blot analysis of membrane, cytosolic, and whole cell lysate fractions. The compounds also showed antioxidant activities in a DPPH assay.

AB - Copaifera lucens n-butanolic fraction (BF) was used as a source of galloylquinic acids, and aerobically incubated with Aspergillus alliaceus ATCC10060, Aspergillus brasiliensis ATCC 16404, and Cunninghamella elegans ATCC 10028b cultures for 60 and 120 h. Out of the three studied filamentous fungi, A. alliaceus ATCC10060 was able to degrade galloylquinic acids into one major metabolite, 3-O-methylgallic acid (M1). The product was identified by 1H NMR, UPLC-MS/MS, and its potential effect on calcium oxalate monohydrate (COM) crystal binding to Madin-Darby canine kidney cells type I surface was studied. Renal cells pretreatment with BF and M1 for 3 h significantly decreased COM crystaladherence at 50 μg/mL and 5 μM, respectively. Moreover, both M1 and BF significantly reduced the surface expression of COM-binding proteins annexin A1 (ANXA1) and heat shock protein 90 (HSP90), respectively as evidenced by Western blot analysis of membrane, cytosolic, and whole cell lysate fractions. The compounds also showed antioxidant activities in a DPPH assay.

KW - Biotransformation

KW - Calcium oxalate monohydrate

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KW - Galloylquinic acids

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