Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium

Martin G Rodriguez-Porcel, Olivier Gheysens, Ramasamy Paulmurugan, Ian Y. Chen, Karen M. Peterson, Jürgen K. Willmann, Joseph C. Wu, Xiangyang Zhu, Lilach O Lerman, Sanjiv S. Gambhir

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Purpose: We tested the hypothesis that modulation of the microenvironment (using antioxidants) will increase stem cell survival in hypoxia and after transplantation to the myocardium. Procedures: Rat cardiomyoblasts were stably transfected with a reporter gene (firefly luciferase) for bioluminescence imaging (BLI). First, we examined the role of oxidative stress in cells under hypoxic conditions. Subsequently, stem cells were transplanted to the myocardium of rats using high-resolution ultrasound, and their survival was monitored daily using BLI. Results: Under hypoxia, oxidative stress was increased together with decreased cell survival compared to control cells, both of which were preserved by antioxidants. In living subjects, oxidative stress blockade increased early cell survival after transplantation to the myocardium, compared to untreated cells/animals. Conclusion: Modulation of the local microenvironment (with antioxidants) improves stem cell survival. Increased understanding of the interaction between stem cells and their microenvironment will be critical to advance the field of regenerative medicine.

Original languageEnglish (US)
Pages (from-to)325-334
Number of pages10
JournalMolecular Imaging and Biology
Volume12
Issue number3
DOIs
StatePublished - Jun 2010

Fingerprint

Cell Survival
Myocardium
Antioxidants
Transplantation
Oxidative Stress
Stem Cells
Stem Cell Niche
Firefly Luciferases
Regenerative Medicine
Reporter Genes
Hypoxia

Keywords

  • Antioxidants
  • Bioluminescence
  • Firefly luciferase
  • Molecular imaging
  • Myoblasts
  • Myocardium
  • Oxidative stress
  • Stem cell

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Medicine(all)

Cite this

Rodriguez-Porcel, M. G., Gheysens, O., Paulmurugan, R., Chen, I. Y., Peterson, K. M., Willmann, J. K., ... Gambhir, S. S. (2010). Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium. Molecular Imaging and Biology, 12(3), 325-334. https://doi.org/10.1007/s11307-009-0274-4

Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium. / Rodriguez-Porcel, Martin G; Gheysens, Olivier; Paulmurugan, Ramasamy; Chen, Ian Y.; Peterson, Karen M.; Willmann, Jürgen K.; Wu, Joseph C.; Zhu, Xiangyang; Lerman, Lilach O; Gambhir, Sanjiv S.

In: Molecular Imaging and Biology, Vol. 12, No. 3, 06.2010, p. 325-334.

Research output: Contribution to journalArticle

Rodriguez-Porcel, MG, Gheysens, O, Paulmurugan, R, Chen, IY, Peterson, KM, Willmann, JK, Wu, JC, Zhu, X, Lerman, LO & Gambhir, SS 2010, 'Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium', Molecular Imaging and Biology, vol. 12, no. 3, pp. 325-334. https://doi.org/10.1007/s11307-009-0274-4
Rodriguez-Porcel, Martin G ; Gheysens, Olivier ; Paulmurugan, Ramasamy ; Chen, Ian Y. ; Peterson, Karen M. ; Willmann, Jürgen K. ; Wu, Joseph C. ; Zhu, Xiangyang ; Lerman, Lilach O ; Gambhir, Sanjiv S. / Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium. In: Molecular Imaging and Biology. 2010 ; Vol. 12, No. 3. pp. 325-334.
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