Abstract
Purpose: We tested the hypothesis that modulation of the microenvironment (using antioxidants) will increase stem cell survival in hypoxia and after transplantation to the myocardium. Procedures: Rat cardiomyoblasts were stably transfected with a reporter gene (firefly luciferase) for bioluminescence imaging (BLI). First, we examined the role of oxidative stress in cells under hypoxic conditions. Subsequently, stem cells were transplanted to the myocardium of rats using high-resolution ultrasound, and their survival was monitored daily using BLI. Results: Under hypoxia, oxidative stress was increased together with decreased cell survival compared to control cells, both of which were preserved by antioxidants. In living subjects, oxidative stress blockade increased early cell survival after transplantation to the myocardium, compared to untreated cells/animals. Conclusion: Modulation of the local microenvironment (with antioxidants) improves stem cell survival. Increased understanding of the interaction between stem cells and their microenvironment will be critical to advance the field of regenerative medicine.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 325-334 |
| Number of pages | 10 |
| Journal | Molecular Imaging and Biology |
| Volume | 12 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 2010 |
Fingerprint
Keywords
- Antioxidants
- Bioluminescence
- Firefly luciferase
- Molecular imaging
- Myoblasts
- Myocardium
- Oxidative stress
- Stem cell
ASJC Scopus subject areas
- Cancer Research
- Oncology
- Radiology Nuclear Medicine and imaging
- Medicine(all)
Cite this
Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium. / Rodriguez-Porcel, Martin G; Gheysens, Olivier; Paulmurugan, Ramasamy; Chen, Ian Y.; Peterson, Karen M.; Willmann, Jürgen K.; Wu, Joseph C.; Zhu, Xiangyang; Lerman, Lilach O; Gambhir, Sanjiv S.
In: Molecular Imaging and Biology, Vol. 12, No. 3, 06.2010, p. 325-334.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antioxidants improve early survival of cardiomyoblasts after transplantation to the myocardium
AU - Rodriguez-Porcel, Martin G
AU - Gheysens, Olivier
AU - Paulmurugan, Ramasamy
AU - Chen, Ian Y.
AU - Peterson, Karen M.
AU - Willmann, Jürgen K.
AU - Wu, Joseph C.
AU - Zhu, Xiangyang
AU - Lerman, Lilach O
AU - Gambhir, Sanjiv S.
PY - 2010/6
Y1 - 2010/6
N2 - Purpose: We tested the hypothesis that modulation of the microenvironment (using antioxidants) will increase stem cell survival in hypoxia and after transplantation to the myocardium. Procedures: Rat cardiomyoblasts were stably transfected with a reporter gene (firefly luciferase) for bioluminescence imaging (BLI). First, we examined the role of oxidative stress in cells under hypoxic conditions. Subsequently, stem cells were transplanted to the myocardium of rats using high-resolution ultrasound, and their survival was monitored daily using BLI. Results: Under hypoxia, oxidative stress was increased together with decreased cell survival compared to control cells, both of which were preserved by antioxidants. In living subjects, oxidative stress blockade increased early cell survival after transplantation to the myocardium, compared to untreated cells/animals. Conclusion: Modulation of the local microenvironment (with antioxidants) improves stem cell survival. Increased understanding of the interaction between stem cells and their microenvironment will be critical to advance the field of regenerative medicine.
AB - Purpose: We tested the hypothesis that modulation of the microenvironment (using antioxidants) will increase stem cell survival in hypoxia and after transplantation to the myocardium. Procedures: Rat cardiomyoblasts were stably transfected with a reporter gene (firefly luciferase) for bioluminescence imaging (BLI). First, we examined the role of oxidative stress in cells under hypoxic conditions. Subsequently, stem cells were transplanted to the myocardium of rats using high-resolution ultrasound, and their survival was monitored daily using BLI. Results: Under hypoxia, oxidative stress was increased together with decreased cell survival compared to control cells, both of which were preserved by antioxidants. In living subjects, oxidative stress blockade increased early cell survival after transplantation to the myocardium, compared to untreated cells/animals. Conclusion: Modulation of the local microenvironment (with antioxidants) improves stem cell survival. Increased understanding of the interaction between stem cells and their microenvironment will be critical to advance the field of regenerative medicine.
KW - Antioxidants
KW - Bioluminescence
KW - Firefly luciferase
KW - Molecular imaging
KW - Myoblasts
KW - Myocardium
KW - Oxidative stress
KW - Stem cell
UR - http://www.scopus.com/inward/record.url?scp=77956680293&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956680293&partnerID=8YFLogxK
U2 - 10.1007/s11307-009-0274-4
DO - 10.1007/s11307-009-0274-4
M3 - Article
C2 - 20013064
AN - SCOPUS:77956680293
VL - 12
SP - 325
EP - 334
JO - Molecular Imaging and Biology
JF - Molecular Imaging and Biology
SN - 1536-1632
IS - 3
ER -