Antimicrobial susceptibility and biofilm formation of Staphylococcus epidermidis small colony variants associated with prosthetic joint infection

Awele N. Maduka-Ezeh, Kerryl E. Greenwood-Quaintance, Melissa J. Karau, Elie F. Berbari, Douglas R. Osmon, Arlen D. Hanssen, James M. Steckelberg, Robin Patel

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We determined the frequency of isolation of non- aureus staphylococcal small colony variants (SCVs) from 31 patients with staphylococcal prosthetic joint infection (PJI) and described the antimicrobial susceptibility, auxotrophy, and biofilm-forming capacity of these SCVs. Eleven non- aureus SCVs were recovered, all of which were Staphylococcus epidermidis, and none of which was auxotrophic for hemin, menadione, or thymidine. Aminoglycoside resistance was detected in 5. Two were proficient, and 7 were poor, biofilm formers. With passage on antimicrobial free media, we observed a fluctuating phenotype in 3 isolates. We also noted a difference in antimicrobial susceptibility of different morphology isolates recovered from the same joints despite similar pulsed-field gel electrophoresis patterns. Our findings suggest S. epidermidis SCVs are common in PJI, and while they have a similar appearance to S. aureus SCVs, they do not necessarily share such characteristics as aminoglycoside resistance; auxotrophy for hemin, menadione, or thymidine; or enhanced biofilm formation. We also underscore the importance of antimicrobial susceptibility testing of all morphologies of isolates recovered from PJI.

Original languageEnglish (US)
Pages (from-to)224-229
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume74
Issue number3
DOIs
StatePublished - Nov 2012

Fingerprint

Staphylococcus epidermidis
Biofilms
Joints
Vitamin K 3
Hemin
Aminoglycosides
Infection
Thymidine
Pulsed Field Gel Electrophoresis
Phenotype

Keywords

  • Small colony variant
  • Staphylococcus epidermidis

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Antimicrobial susceptibility and biofilm formation of Staphylococcus epidermidis small colony variants associated with prosthetic joint infection. / Maduka-Ezeh, Awele N.; Greenwood-Quaintance, Kerryl E.; Karau, Melissa J.; Berbari, Elie F.; Osmon, Douglas R.; Hanssen, Arlen D.; Steckelberg, James M.; Patel, Robin.

In: Diagnostic Microbiology and Infectious Disease, Vol. 74, No. 3, 11.2012, p. 224-229.

Research output: Contribution to journalArticle

Maduka-Ezeh, Awele N. ; Greenwood-Quaintance, Kerryl E. ; Karau, Melissa J. ; Berbari, Elie F. ; Osmon, Douglas R. ; Hanssen, Arlen D. ; Steckelberg, James M. ; Patel, Robin. / Antimicrobial susceptibility and biofilm formation of Staphylococcus epidermidis small colony variants associated with prosthetic joint infection. In: Diagnostic Microbiology and Infectious Disease. 2012 ; Vol. 74, No. 3. pp. 224-229.
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