Antibodies to iris and retina detected in sera from patients with juvenile rheumatoid arthritis with iridocyclitis by indirect immunofluorescence studies on human eye tissue

R. C. Uchiyama, Thomas Osborn, T. L. Moore

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Sera from 12 patients with juvenile rheumatoid arthritis (JRA) with active iridocyclitis were incubated with frozen, sectioned, whole human eye tissue. Antibodies were detected using fluorescein conjugated goat F(ab')2 antibody to human IgG by immunofluorescent microscopy. Immunofluorescence was determined on tissue from the iris, retina, and 3 portions of the ciliary body. Sera of patients with JRA with iridocyclitis were compared to sera from patients with JRA with and without antinuclear antibodies (ANA) and healthy children. An increased frequency of antibody to the human iris was seen with sera of patients with JRA with iridocyclitis compared to healthy children's sera. A higher frequency of antibody was also noted to human retina in sera of patients with JRA with iridocyclitis compared to patients without ANA and healthy children. No increased frequency of antibody was detected to ciliary body. Sera of 7 patients with JRA with iridocyclitis were also compared during a time of inactive eye disease to a time of active disease. No difference in binding to eye tissue was detected at times of inactive disease compared to controls. Our results demonstrate the presence of antibody to iris and retina by immunofluorescence in the sera of patients with JRA with iridocyclitis.

Original languageEnglish (US)
Pages (from-to)1074-1078
Number of pages5
JournalJournal of Rheumatology
Volume16
Issue number8
StatePublished - Jan 1 1989
Externally publishedYes

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Iridocyclitis
Juvenile Arthritis
Iris
Indirect Fluorescent Antibody Technique
Retina
Antibodies
Serum
Ciliary Body
Antinuclear Antibodies
Fluorescent Antibody Technique
Eye Diseases
Fluorescein
Goats
Microscopy
Immunoglobulin G

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

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abstract = "Sera from 12 patients with juvenile rheumatoid arthritis (JRA) with active iridocyclitis were incubated with frozen, sectioned, whole human eye tissue. Antibodies were detected using fluorescein conjugated goat F(ab')2 antibody to human IgG by immunofluorescent microscopy. Immunofluorescence was determined on tissue from the iris, retina, and 3 portions of the ciliary body. Sera of patients with JRA with iridocyclitis were compared to sera from patients with JRA with and without antinuclear antibodies (ANA) and healthy children. An increased frequency of antibody to the human iris was seen with sera of patients with JRA with iridocyclitis compared to healthy children's sera. A higher frequency of antibody was also noted to human retina in sera of patients with JRA with iridocyclitis compared to patients without ANA and healthy children. No increased frequency of antibody was detected to ciliary body. Sera of 7 patients with JRA with iridocyclitis were also compared during a time of inactive eye disease to a time of active disease. No difference in binding to eye tissue was detected at times of inactive disease compared to controls. Our results demonstrate the presence of antibody to iris and retina by immunofluorescence in the sera of patients with JRA with iridocyclitis.",
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