TY - JOUR
T1 - Antiangiogenic properties of gold nanoparticles
AU - Mukherjee, Priyabrata
AU - Bhattacharya, Resham
AU - Wang, Ping
AU - Wang, Ling
AU - Basu, Sujit
AU - Nagy, Janice A.
AU - Atala, Anthony
AU - Mukhopadhyay, Debabrata
AU - Soker, Shay
PY - 2005/5/1
Y1 - 2005/5/1
N2 - Here, we report an intrinsic property of gold nanoparticles (nanogold): they can interact selectively with heparin-binding glycoproteins and inhibit their activity. Gold nanoparticles specifically bound vascular permeability factor/vascular endothelial growth factor (VPF/VEGF)-165 and basic fibroblast growth factor, two endothetial cell mitogens and mediators of angiogenesis resulting in inhibition of endothelial/fibroblast cell proliferation in vitro and VEGF-induced permeability as well as angiogenesis in vivo. In contrast, nanogold did not inhibit VEGF-121 or epidermal growth factor, two non-heparin-binding growth factors, mediated cell proliferation. Gold nanoparticles significantly inhibited VEGF receptor-2 phosphorylation, intracellular calcium release, and migration and RhoA activation in vitro. These results report for the first time a novel property of gold nanoparticles to bind heparin-binding proteins and thereby inhibit their subsequent signaling events.
AB - Here, we report an intrinsic property of gold nanoparticles (nanogold): they can interact selectively with heparin-binding glycoproteins and inhibit their activity. Gold nanoparticles specifically bound vascular permeability factor/vascular endothelial growth factor (VPF/VEGF)-165 and basic fibroblast growth factor, two endothetial cell mitogens and mediators of angiogenesis resulting in inhibition of endothelial/fibroblast cell proliferation in vitro and VEGF-induced permeability as well as angiogenesis in vivo. In contrast, nanogold did not inhibit VEGF-121 or epidermal growth factor, two non-heparin-binding growth factors, mediated cell proliferation. Gold nanoparticles significantly inhibited VEGF receptor-2 phosphorylation, intracellular calcium release, and migration and RhoA activation in vitro. These results report for the first time a novel property of gold nanoparticles to bind heparin-binding proteins and thereby inhibit their subsequent signaling events.
UR - http://www.scopus.com/inward/record.url?scp=18244396366&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=18244396366&partnerID=8YFLogxK
U2 - 10.1158/1078-0432.CCR-04-2482
DO - 10.1158/1078-0432.CCR-04-2482
M3 - Article
C2 - 15867256
AN - SCOPUS:18244396366
SN - 1078-0432
VL - 11
SP - 3530
EP - 3534
JO - Clinical Cancer Research
JF - Clinical Cancer Research
IS - 9
ER -