ANP secretion from small cell lung cancer cell lines: A potential model of ANP release

Dennis A Wigle, B. G. Campling, I. R. Sarda, S. H. Shin, J. D. Watson, Y. Frater, T. G. Flynn, S. C. Pang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Although atrial distension is widely accepted as the primary stimulus for atrial natriuretic peptide (ANP) release, a number of agonists are also known to induce its secretion. The mechanisms underlying these processes are not well understood. Studies of this nature are hampered by the inherent difficulty in culturing homogeneous populations of cardiac myocytes in sufficient quantities to perform molecular investigations. For this reason, we have examined the possibility of using other cell types as a model of ANP release. It has been reported that a number of tumor samples from small cell lung cancer (SCLC) patients express the ANP gene. Characterization of a large number of cell lines derived from SCLC tumor samples indicated that two of these cell lines, OS-A and SHP-77, secrete ANP at rates of ~10-20 g · cell-1 · min-1. This is a sufficient quantity to facilitate secretion studies using a perifusion system. We have demonstrated that ANP is released through regulated secretory pathways, as the Ca2+ ionophore A-23187, arginine vasopressin (AVP), and the sodium ionophore, monensin, were capable of modifying secretion rates. High-pressure liquid chromatography (HPLC) analysis indicated that the primary secretory product is ANP-(99-126), the circulating form of this hormone. Intracellularly, both ANP-(99-126) and ANP- (1-126) were present, suggesting the synthesis and appropriate cleavage of pro-ANP-(1-126). Because both of these cell lines have doubling times in the range of 3-5 days, they could serve as a rapidly proliferating and easily maintainable supply of homogeneous tissue for release studies. The two identified SCLC cell lines may provide a unique opportunity to investigate the molecular events associated with ANP secretion.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume268
Issue number5 37-5
StatePublished - 1995
Externally publishedYes

Fingerprint

Small Cell Lung Carcinoma
Atrial Natriuretic Factor
Cell Line
Sodium Ionophores
Monensin
Arginine Vasopressin
Secretory Pathway
Ionophores
Calcimycin
Cardiac Myocytes
Neoplasms
High Pressure Liquid Chromatography
Hormones

Keywords

  • arginine vasopressin
  • atrial natriuretic peptide release
  • calcium ionophore A-23187
  • perifusion technique
  • sodium ionophore monensin

ASJC Scopus subject areas

  • Physiology

Cite this

Wigle, D. A., Campling, B. G., Sarda, I. R., Shin, S. H., Watson, J. D., Frater, Y., ... Pang, S. C. (1995). ANP secretion from small cell lung cancer cell lines: A potential model of ANP release. American Journal of Physiology - Heart and Circulatory Physiology, 268(5 37-5).

ANP secretion from small cell lung cancer cell lines : A potential model of ANP release. / Wigle, Dennis A; Campling, B. G.; Sarda, I. R.; Shin, S. H.; Watson, J. D.; Frater, Y.; Flynn, T. G.; Pang, S. C.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 268, No. 5 37-5, 1995.

Research output: Contribution to journalArticle

Wigle, DA, Campling, BG, Sarda, IR, Shin, SH, Watson, JD, Frater, Y, Flynn, TG & Pang, SC 1995, 'ANP secretion from small cell lung cancer cell lines: A potential model of ANP release', American Journal of Physiology - Heart and Circulatory Physiology, vol. 268, no. 5 37-5.
Wigle, Dennis A ; Campling, B. G. ; Sarda, I. R. ; Shin, S. H. ; Watson, J. D. ; Frater, Y. ; Flynn, T. G. ; Pang, S. C. / ANP secretion from small cell lung cancer cell lines : A potential model of ANP release. In: American Journal of Physiology - Heart and Circulatory Physiology. 1995 ; Vol. 268, No. 5 37-5.
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AU - Shin, S. H.

AU - Watson, J. D.

AU - Frater, Y.

AU - Flynn, T. G.

AU - Pang, S. C.

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