TY - JOUR
T1 - Angiogenic factor
T2 - A possible mechanism for neovascularization produced by omental pedicles
AU - Cartier, R.
AU - Brunette, I.
AU - Hashimoto, K.
AU - Bourne, W. M.
AU - Schaff, H. V.
PY - 1990
Y1 - 1990
N2 - To determine possible mechanisms by which omental pedicles protect bronchial anastomoses from ischemia, we studied the angiogenic potential of a lipid extract of omentum. A rabbit cornea model was used to quantify neovascularization produced by methanol-chloroform extract of homogenized autologous omentum or perirenal fat. In 22 anesthetized rabbits, 10 μl of omental lipid extract was injected into the cornea. In each animal the opposite eye was used as a control and was injected with a similar volume of extract prepared from perirenal fat. The side of injection of autologous omental fat was randomized and was not known to the investigator who assessed neovascularization on days 4, 7, 14, and 21 after injection. Neovascularization was recorded on microphotography and quantified by a point-counting method. Four days after injection, neovascularization in corneas injected with autologous omental fat was significantly (p < 0.05) greater than in control corneas for all indices of neovascularization including total point count, which was three times greater than control. The angiogenic effect diminished with time, and by 21 days after injection corneal neovascularization was comparable for the two groups. Our results suggest that the lipid fraction of omentum has angiogenic activity that may stimulate neovascularization in ischemic tissues. Lack of sustained activity may be due to washout by neovessels or local tissue metabolism.
AB - To determine possible mechanisms by which omental pedicles protect bronchial anastomoses from ischemia, we studied the angiogenic potential of a lipid extract of omentum. A rabbit cornea model was used to quantify neovascularization produced by methanol-chloroform extract of homogenized autologous omentum or perirenal fat. In 22 anesthetized rabbits, 10 μl of omental lipid extract was injected into the cornea. In each animal the opposite eye was used as a control and was injected with a similar volume of extract prepared from perirenal fat. The side of injection of autologous omental fat was randomized and was not known to the investigator who assessed neovascularization on days 4, 7, 14, and 21 after injection. Neovascularization was recorded on microphotography and quantified by a point-counting method. Four days after injection, neovascularization in corneas injected with autologous omental fat was significantly (p < 0.05) greater than in control corneas for all indices of neovascularization including total point count, which was three times greater than control. The angiogenic effect diminished with time, and by 21 days after injection corneal neovascularization was comparable for the two groups. Our results suggest that the lipid fraction of omentum has angiogenic activity that may stimulate neovascularization in ischemic tissues. Lack of sustained activity may be due to washout by neovessels or local tissue metabolism.
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U2 - 10.1016/s0022-5223(19)37010-2
DO - 10.1016/s0022-5223(19)37010-2
M3 - Article
C2 - 1688990
AN - SCOPUS:0025347153
SN - 0022-5223
VL - 99
SP - 264
EP - 268
JO - Journal of Thoracic and Cardiovascular Surgery
JF - Journal of Thoracic and Cardiovascular Surgery
IS - 2
ER -