OBJECTIVE: To establish and optimize two-dimensional (2-D) polyacrylamide gel electrophoresis (PAGE) for comparative analysis of the protein expression profiles in mouse fibroblast NIH3T3 cells before and after 12-O-tetradecanoy- lphorbol-13-acetate (TPA) treatment using image analysis software, so as to prepare for more intensive study in the identification of the proteins that mediate the biological effect of TPA. METHODS: The total cellular protein extracted from NIH3T3 cells with or without TPA treatment underwent 2-D PAGE and silver nitrate staining prior to the analysis of the differential protein expressions using image analysis software. RESULTS: Image analysis revealed obvious differential protein expressions of the cells in response to TPA treatment. CONCLUSION: High-quality instruments for 2-D PAGE, skilled electrophoretic operation and efficient image processing are all essential in the reliable identification of stable functional proteins.
|Original language||English (US)|
|Number of pages||4|
|Journal||Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA|
|State||Published - Mar 2004|
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