Analysis of the cellular and molecular mechanisms of trophic action of a misspliced form of the type B cholecystokinin receptor present in colon and pancreatic cancer

Zhi Jie Cheng, Kaleeckal G. Harikumar, Wei Qun Ding, Eileen L. Holicky, Laurence J Miller

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Gastrin and cholecystokinin (CCK) have trophic action on cells expressing wild type A or B CCK receptors. Potential relevance to pancreatic and colonic cancers was raised by the demonstration of a misspliced type B CCK receptor that, when expressed in Balb3T3 cells, had constitutive activity to stimulate intracellular calcium. We attempted to confirm and extend this observation in CHO cells by establishing lines expressing similar densities of variant or wild type B CCK receptor. While both were capable of normal binding and agonist-induced signaling, neither expressed constitutive signaling and both had similar basal growth. Agonist stimulation of cells expressing misspliced receptor had greater increases in calcium and greater growth rates than control cells despite similar MAP kinase phosphorylation responses. Thus, this variant receptor can potentate peptide-stimulated signaling and trophic action and may contribute to the proliferation of neoplasms expressing it.

Original languageEnglish (US)
Pages (from-to)95-105
Number of pages11
JournalCancer Letters
Volume222
Issue number1
DOIs
StatePublished - May 10 2005

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Keywords

  • Cholecystokinin
  • G protein-coupled receptor
  • Gastrin
  • Peptide hormone
  • Signaling

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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