Analysis of protein expression maps of two metastatic colorectal carcinoma cell lines generated by two-dimensional gel electrophoresis

L. Liang, Yan qing Ding, Xin Li, Pei zhou Jiang, Xue hua Li, Jin hua Zhang

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To analyze the differentially expressed proteins in metastatic colorectal carcinoma and provide clues for the molecular mechanisms of colorectal cancer metastasis. METHODS: A pair of colorectal carcinoma cell lines SW620 and SW480 with different metastatic potentials from the same parent cell lines were studied. The protein expression maps of the two cell lines were obtained and optimized using two-dimensional gel electrophoresis (2-DE), and the differentially expressed protein spots associated with metastasis were analyzed by Melanie III software. RESULTS: The 2-DE expression maps of the two cell lines were well matched. The resolution of 2-DE was increased after using the nonlinear pH3-10 or overlapping narrow immobilized pH gradient strips, and 11 differentially expressed protein spots were identified after analysis using the software. CONCLUSION: Nonlinear pH3-10 and overlapping narrow immobilized pH gradient strips have higher separation efficiency for protein in 2-DE. The differentially expressed proteins separated will be useful for identifying metastasis-associated proteins of colorectal carcinoma and for establishing 2-DE database of the metastatic colorectal carcinoma cell lines.

Original languageEnglish (US)
Pages (from-to)18-21
Number of pages4
JournalDi 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
Volume25
Issue number1
StatePublished - Jan 2005

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ASJC Scopus subject areas

  • Medicine(all)

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