Analysis of human sodium iodide symporter immunoreactivity in human exocrine glands

Christine Spitzweg, W. Joba, K. Schriever, J. R. Goellner, J. C. Morris, A. E. Heufelder

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The human sodium iodide symporter (hNIS) is an intrinsic transmembrane protein that mediates the active transport of iodide across the basolateral membrane of thyroid follicular cells. In addition to normally functioning thyroid tissue, various extrathyroidal tissues, including salivary gland, lacrimal gland, gastric mucosa, choroid plexus, and lactating mammary gland, have been demonstrated to accumulate iodide. After cloning and molecular characterization of the sodium iodide symporter, expression of hNIS messenger ribonucleic acid has been detected in a broad range of extrathyroidal tissues using Northern blot analysis and RT-PCR. In this study we used both monoclonal and polyclonal antibodies directed against different portions of hNIS protein together with a highly sensitive immanostaining technique to assess hNIS protein expression in tissue sections derived from normal human salivary and lacrimal glands, pancreas, as well as gastric and colonic mucosa. Immunohistochemical analysis of normal human salivary and lacrimal glands revealed marked hNIS immunoreactivity in ductal cells and less intense staining of acinar cells. Further, immunostaining of gastric and colonic mucosa showed marked hNIS immunoreactivity confined to chief and parietal cells in gastric mucosa and to epithelial cells lining mucosal crypts in colonic mucosa. In normal human pancreas, hNIS immuno-reactivity was located in ductal cells, exocrine parenchymal cells, and Langerhans islet cells. In conclusion, our study demonstrates the expression of hNIS protein by several human exocrine glands, suggesting that iodide transport in these glands is a specific property conferred by the expression of hNIS protein, which may serve important functions by concentrating iodine in glandular secretions.

Original languageEnglish (US)
Pages (from-to)4178-4184
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume84
Issue number11
StatePublished - 1999

Fingerprint

Exocrine Glands
Iodides
Gastric Mucosa
Lacrimal Apparatus
Tissue
Salivary Glands
Proteins
sodium-iodide symporter
Pancreas
Cloning
Linings
Iodine
Choroid Plexus
Langerhans Cells
Acinar Cells
Active Biological Transport
Molecular Cloning
Human Mammary Glands
Islets of Langerhans
Northern Blotting

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Medicine(all)
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Spitzweg, C., Joba, W., Schriever, K., Goellner, J. R., Morris, J. C., & Heufelder, A. E. (1999). Analysis of human sodium iodide symporter immunoreactivity in human exocrine glands. Journal of Clinical Endocrinology and Metabolism, 84(11), 4178-4184.

Analysis of human sodium iodide symporter immunoreactivity in human exocrine glands. / Spitzweg, Christine; Joba, W.; Schriever, K.; Goellner, J. R.; Morris, J. C.; Heufelder, A. E.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 84, No. 11, 1999, p. 4178-4184.

Research output: Contribution to journalArticle

Spitzweg, C, Joba, W, Schriever, K, Goellner, JR, Morris, JC & Heufelder, AE 1999, 'Analysis of human sodium iodide symporter immunoreactivity in human exocrine glands', Journal of Clinical Endocrinology and Metabolism, vol. 84, no. 11, pp. 4178-4184.
Spitzweg C, Joba W, Schriever K, Goellner JR, Morris JC, Heufelder AE. Analysis of human sodium iodide symporter immunoreactivity in human exocrine glands. Journal of Clinical Endocrinology and Metabolism. 1999;84(11):4178-4184.
Spitzweg, Christine ; Joba, W. ; Schriever, K. ; Goellner, J. R. ; Morris, J. C. ; Heufelder, A. E. / Analysis of human sodium iodide symporter immunoreactivity in human exocrine glands. In: Journal of Clinical Endocrinology and Metabolism. 1999 ; Vol. 84, No. 11. pp. 4178-4184.
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