Analysis of blood T-cell cytokine expression in B-chronic lymphocytic leukaemia: Evidence for increased levels of cytoplasmic IL-4 in resting and activated CD8 T cells

Xi Mu, Neil E. Kay, Michael P. Gosland, C. Darrell Jennings

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

The cytoplasmic cytokines of purified blood T cells (CD4/CD8) in B-CLL patients (n = 5) and controls (n = 5) were evaluated by flow cytometry. The mean levels of cytoplasmic IL-4 were significantly elevated in resting and activated B-CLL CD8 cells compared to control CD8 cells. IL-4 cytoplasmic levels were comparable for resting B-CLL and control CD4 cells but greater for B-CLL activated CD4 cells. The mean fluorescence intensity of B-CLL CD8 cytoplasmic IL-4 was 4-5-fold greater, indicating higher IL-4 density per CLL CD8 than control CD8 cells. Both CLL CD4 and CD8 cells post-activation had higher levels of cells double positive for cytoplasmic IL-4 and interferon. These data indicate that freshly isolated CD8 and CD4 blood T cells from B-CLL patients have significantly elevated (above control) levels of commitment to expression of IL-4. Since IL-4 has an important modulatory impact on CLL and normal B cells, this observation has implications regarding the biology of B-CLL.

Original languageEnglish (US)
Pages (from-to)733-735
Number of pages3
JournalBritish journal of haematology
Volume96
Issue number4
DOIs
StatePublished - Jan 1 1997

Keywords

  • IL-4
  • T lymphocytes
  • chronic lymphocyte leukaemia
  • cytokines
  • immunoregulation

ASJC Scopus subject areas

  • Hematology

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