Analysis of blood T-cell cytokine expression in B-chronic lymphocytic leukaemia

Evidence for increased levels of cytoplasmic IL-4 in resting and activated CD8 T cells

Xi Mu, Neil Elliot Kay, Michael P. Gosland, C. Darrell Jennings

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The cytoplasmic cytokines of purified blood T cells (CD4/CD8) in B-CLL patients (n = 5) and controls (n = 5) were evaluated by flow cytometry. The mean levels of cytoplasmic IL-4 were significantly elevated in resting and activated B-CLL CD8 cells compared to control CD8 cells. IL-4 cytoplasmic levels were comparable for resting B-CLL and control CD4 cells but greater for B-CLL activated CD4 cells. The mean fluorescence intensity of B-CLL CD8 cytoplasmic IL-4 was 4-5-fold greater, indicating higher IL-4 density per CLL CD8 than control CD8 cells. Both CLL CD4 and CD8 cells post-activation had higher levels of cells double positive for cytoplasmic IL-4 and interferon. These data indicate that freshly isolated CD8 and CD4 blood T cells from B-CLL patients have significantly elevated (above control) levels of commitment to expression of IL-4. Since IL-4 has an important modulatory impact on CLL and normal B cells, this observation has implications regarding the biology of B-CLL.

Original languageEnglish (US)
Pages (from-to)733-735
Number of pages3
JournalBritish Journal of Haematology
Volume96
Issue number4
StatePublished - 1997
Externally publishedYes

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B-Cell Chronic Lymphocytic Leukemia
Interleukin-4
Blood Cells
Cytokines
T-Lymphocytes
Interferons
Flow Cytometry
B-Lymphocytes
Fluorescence

Keywords

  • chronic lymphocyte leukaemia
  • cytokines
  • IL-4
  • immunoregulation
  • T lymphocytes

ASJC Scopus subject areas

  • Hematology

Cite this

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title = "Analysis of blood T-cell cytokine expression in B-chronic lymphocytic leukaemia: Evidence for increased levels of cytoplasmic IL-4 in resting and activated CD8 T cells",
abstract = "The cytoplasmic cytokines of purified blood T cells (CD4/CD8) in B-CLL patients (n = 5) and controls (n = 5) were evaluated by flow cytometry. The mean levels of cytoplasmic IL-4 were significantly elevated in resting and activated B-CLL CD8 cells compared to control CD8 cells. IL-4 cytoplasmic levels were comparable for resting B-CLL and control CD4 cells but greater for B-CLL activated CD4 cells. The mean fluorescence intensity of B-CLL CD8 cytoplasmic IL-4 was 4-5-fold greater, indicating higher IL-4 density per CLL CD8 than control CD8 cells. Both CLL CD4 and CD8 cells post-activation had higher levels of cells double positive for cytoplasmic IL-4 and interferon. These data indicate that freshly isolated CD8 and CD4 blood T cells from B-CLL patients have significantly elevated (above control) levels of commitment to expression of IL-4. Since IL-4 has an important modulatory impact on CLL and normal B cells, this observation has implications regarding the biology of B-CLL.",
keywords = "chronic lymphocyte leukaemia, cytokines, IL-4, immunoregulation, T lymphocytes",
author = "Xi Mu and Kay, {Neil Elliot} and Gosland, {Michael P.} and {Darrell Jennings}, C.",
year = "1997",
language = "English (US)",
volume = "96",
pages = "733--735",
journal = "British Journal of Haematology",
issn = "0007-1048",
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TY - JOUR

T1 - Analysis of blood T-cell cytokine expression in B-chronic lymphocytic leukaemia

T2 - Evidence for increased levels of cytoplasmic IL-4 in resting and activated CD8 T cells

AU - Mu, Xi

AU - Kay, Neil Elliot

AU - Gosland, Michael P.

AU - Darrell Jennings, C.

PY - 1997

Y1 - 1997

N2 - The cytoplasmic cytokines of purified blood T cells (CD4/CD8) in B-CLL patients (n = 5) and controls (n = 5) were evaluated by flow cytometry. The mean levels of cytoplasmic IL-4 were significantly elevated in resting and activated B-CLL CD8 cells compared to control CD8 cells. IL-4 cytoplasmic levels were comparable for resting B-CLL and control CD4 cells but greater for B-CLL activated CD4 cells. The mean fluorescence intensity of B-CLL CD8 cytoplasmic IL-4 was 4-5-fold greater, indicating higher IL-4 density per CLL CD8 than control CD8 cells. Both CLL CD4 and CD8 cells post-activation had higher levels of cells double positive for cytoplasmic IL-4 and interferon. These data indicate that freshly isolated CD8 and CD4 blood T cells from B-CLL patients have significantly elevated (above control) levels of commitment to expression of IL-4. Since IL-4 has an important modulatory impact on CLL and normal B cells, this observation has implications regarding the biology of B-CLL.

AB - The cytoplasmic cytokines of purified blood T cells (CD4/CD8) in B-CLL patients (n = 5) and controls (n = 5) were evaluated by flow cytometry. The mean levels of cytoplasmic IL-4 were significantly elevated in resting and activated B-CLL CD8 cells compared to control CD8 cells. IL-4 cytoplasmic levels were comparable for resting B-CLL and control CD4 cells but greater for B-CLL activated CD4 cells. The mean fluorescence intensity of B-CLL CD8 cytoplasmic IL-4 was 4-5-fold greater, indicating higher IL-4 density per CLL CD8 than control CD8 cells. Both CLL CD4 and CD8 cells post-activation had higher levels of cells double positive for cytoplasmic IL-4 and interferon. These data indicate that freshly isolated CD8 and CD4 blood T cells from B-CLL patients have significantly elevated (above control) levels of commitment to expression of IL-4. Since IL-4 has an important modulatory impact on CLL and normal B cells, this observation has implications regarding the biology of B-CLL.

KW - chronic lymphocyte leukaemia

KW - cytokines

KW - IL-4

KW - immunoregulation

KW - T lymphocytes

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