An SNP selection strategy identified IL-22 associating with susceptibility to tuberculosis in Chinese

Guoliang Zhang; Xinchun Chen; Long Chan; Mingxia Zhang; Baohua Zhu; Lantian Wang; Xiuyun Zhu; Jieyun Zhang; Boping Zhou; Junwen Wang

doi:10.1038/srep00020

An SNP selection strategy identified IL-22 associating with susceptibility to tuberculosis in Chinese

Guoliang Zhang, Xinchun Chen, Long Chan, Mingxia Zhang, Baohua Zhu, Lantian Wang, Xiuyun Zhu, Jieyun Zhang, Boping Zhou, Junwen Wang

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Abstract

Recent studies showed that IL-22 plays a protective role in the host defense. However, the contribution of polymorphisms of the IL-22 gene to human TB susceptibility remains untested. We have designed a computational approach to select functional SNPs in the IL-22 gene and genotyped them in a two-stage case-control study in Chinese (479 cases and 358 controls). We found that rs2227473, an SNP in the promoter region of IL-22, is associated with TB susceptibility at both stages of our study. The SNP shows associations with p-values of 0.028 and 0.034 respectively, and a combined p-value of 0.0086, with odds ratio at 0.65 (95% confidence interval 0.45-0.90). We further validated the association with an independent cohort (413 cases and 241 controls in Chinese). Our functional studies showed that patients with A allele have significantly higher IL-22 expression than those without A allele under both non-specific and specific stimulations.

Original language	English (US)
Article number	20
Journal	Scientific reports
Volume	1
DOIs	https://doi.org/10.1038/srep00020
State	Published - 2011

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title = "An SNP selection strategy identified IL-22 associating with susceptibility to tuberculosis in Chinese",

abstract = "Recent studies showed that IL-22 plays a protective role in the host defense. However, the contribution of polymorphisms of the IL-22 gene to human TB susceptibility remains untested. We have designed a computational approach to select functional SNPs in the IL-22 gene and genotyped them in a two-stage case-control study in Chinese (479 cases and 358 controls). We found that rs2227473, an SNP in the promoter region of IL-22, is associated with TB susceptibility at both stages of our study. The SNP shows associations with p-values of 0.028 and 0.034 respectively, and a combined p-value of 0.0086, with odds ratio at 0.65 (95% confidence interval 0.45-0.90). We further validated the association with an independent cohort (413 cases and 241 controls in Chinese). Our functional studies showed that patients with A allele have significantly higher IL-22 expression than those without A allele under both non-specific and specific stimulations.",

author = "Guoliang Zhang and Xinchun Chen and Long Chan and Mingxia Zhang and Baohua Zhu and Lantian Wang and Xiuyun Zhu and Jieyun Zhang and Boping Zhou and Junwen Wang",

note = "Funding Information: We thank Yan Zhang, Wanling Yang and Yu Lung Lau of the University of Hong Kong for assistance in genotyping samples and critical comments on the manuscript. Financial support was provided by Eleven-Fifth Mega-Scientific Project on {\textquoteleft}{\textquoteleft}prevention and treatment of AIDS, viral hepatitis and other infectious diseases{\textquoteright}{\textquoteright} (2008ZX10003-005,2008ZX10003-012) and National Science Foundation (30872258) of China, and Research Grants Council (778609M, N_HKU752/10) and Research Fund for the Control of Infectious Diseases (10091262) of Hong Kong.",

year = "2011",

doi = "10.1038/srep00020",

language = "English (US)",

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journal = "Scientific reports",

issn = "2045-2322",

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T1 - An SNP selection strategy identified IL-22 associating with susceptibility to tuberculosis in Chinese

AU - Zhang, Guoliang

AU - Chen, Xinchun

AU - Chan, Long

AU - Zhang, Mingxia

AU - Zhu, Baohua

AU - Wang, Lantian

AU - Zhu, Xiuyun

AU - Zhang, Jieyun

AU - Zhou, Boping

AU - Wang, Junwen

N1 - Funding Information: We thank Yan Zhang, Wanling Yang and Yu Lung Lau of the University of Hong Kong for assistance in genotyping samples and critical comments on the manuscript. Financial support was provided by Eleven-Fifth Mega-Scientific Project on ‘‘prevention and treatment of AIDS, viral hepatitis and other infectious diseases’’ (2008ZX10003-005,2008ZX10003-012) and National Science Foundation (30872258) of China, and Research Grants Council (778609M, N_HKU752/10) and Research Fund for the Control of Infectious Diseases (10091262) of Hong Kong.

PY - 2011

Y1 - 2011

N2 - Recent studies showed that IL-22 plays a protective role in the host defense. However, the contribution of polymorphisms of the IL-22 gene to human TB susceptibility remains untested. We have designed a computational approach to select functional SNPs in the IL-22 gene and genotyped them in a two-stage case-control study in Chinese (479 cases and 358 controls). We found that rs2227473, an SNP in the promoter region of IL-22, is associated with TB susceptibility at both stages of our study. The SNP shows associations with p-values of 0.028 and 0.034 respectively, and a combined p-value of 0.0086, with odds ratio at 0.65 (95% confidence interval 0.45-0.90). We further validated the association with an independent cohort (413 cases and 241 controls in Chinese). Our functional studies showed that patients with A allele have significantly higher IL-22 expression than those without A allele under both non-specific and specific stimulations.

AB - Recent studies showed that IL-22 plays a protective role in the host defense. However, the contribution of polymorphisms of the IL-22 gene to human TB susceptibility remains untested. We have designed a computational approach to select functional SNPs in the IL-22 gene and genotyped them in a two-stage case-control study in Chinese (479 cases and 358 controls). We found that rs2227473, an SNP in the promoter region of IL-22, is associated with TB susceptibility at both stages of our study. The SNP shows associations with p-values of 0.028 and 0.034 respectively, and a combined p-value of 0.0086, with odds ratio at 0.65 (95% confidence interval 0.45-0.90). We further validated the association with an independent cohort (413 cases and 241 controls in Chinese). Our functional studies showed that patients with A allele have significantly higher IL-22 expression than those without A allele under both non-specific and specific stimulations.

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An SNP selection strategy identified IL-22 associating with susceptibility to tuberculosis in Chinese

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