TY - JOUR
T1 - An assessment of the usefulness of immunohistochemical stains in the diagnosis of hairy cell leukemia
AU - Sherman, Michael J.
AU - Hanson, Curtis A.
AU - Hoyer, James D.
PY - 2011/9
Y1 - 2011/9
N2 - Annexin-1 and T-bet are recently described immunohistochemical stains that reportedly assist in the diagnosis of hairy cell leukemia (HCL). Our objective was to assess the sensitivity and specificity of a panel of immunohistochemical stains in distinguishing HCL from other B-cell neoplasms, particularly splenic and extranodal marginal zone lymphomas (SMZL and ENMZL, respectively). The study included 234 bone marrow biopsy specimens: 101 HCL, 13 SMZL, and 10 ENMZL cases were assessed with CD20, tartrate-resistant acid phosphatase (TRAP), DBA.44, a-1, T-bet, and cyclin D1, and 110 control cases were assessed with annexin-1 and T-bet. Our study showed that annexin-1 is a specific and sensitive marker for HCL; however, interpretation is limited by positivity within myeloid precursors. T-bet, DBA.44, and TRAP immunohistochemical stains lack sufficient sensitivity and specificity to differentiate HCL from either form of marginal zone lymphoma. However, our data suggest that the addition cyclin D1 to the immunostaining panel will increase the sensitivity and specificity of HCL diagnosis.
AB - Annexin-1 and T-bet are recently described immunohistochemical stains that reportedly assist in the diagnosis of hairy cell leukemia (HCL). Our objective was to assess the sensitivity and specificity of a panel of immunohistochemical stains in distinguishing HCL from other B-cell neoplasms, particularly splenic and extranodal marginal zone lymphomas (SMZL and ENMZL, respectively). The study included 234 bone marrow biopsy specimens: 101 HCL, 13 SMZL, and 10 ENMZL cases were assessed with CD20, tartrate-resistant acid phosphatase (TRAP), DBA.44, a-1, T-bet, and cyclin D1, and 110 control cases were assessed with annexin-1 and T-bet. Our study showed that annexin-1 is a specific and sensitive marker for HCL; however, interpretation is limited by positivity within myeloid precursors. T-bet, DBA.44, and TRAP immunohistochemical stains lack sufficient sensitivity and specificity to differentiate HCL from either form of marginal zone lymphoma. However, our data suggest that the addition cyclin D1 to the immunostaining panel will increase the sensitivity and specificity of HCL diagnosis.
KW - Annexin-1
KW - Cyclin-D1
KW - Hairy cell leukemia
KW - Immunohistochemistry
KW - Marginal zone lymphoma
KW - T-bet
UR - http://www.scopus.com/inward/record.url?scp=80052658950&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052658950&partnerID=8YFLogxK
U2 - 10.1309/AJCP5GE1PSBMBZTW
DO - 10.1309/AJCP5GE1PSBMBZTW
M3 - Article
C2 - 21846914
AN - SCOPUS:80052658950
SN - 0002-9173
VL - 136
SP - 390
EP - 399
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 3
ER -