An aptasensor for electrochemical detection of tumor necrosis factor in human blood

Ying Liu, Qing Zhou, Alexander Revzin

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Electrochemical aptasensors can detect disease markers such as cytokines to provide point-of-care diagnosis that is low-cost, rapid, specific and sensitive. Herein, we describe the development of an aptamer-based electrochemical sensor for detection and analysis of tumor necrosis factor-alpha (TNF-α)-a key inflammatory cytokine-in whole human blood. When testing spiked blood, a TNF-α detection limit of 58 pM (10 ng mL-1) and a linear range of 6 nM (100 ng mL-1) could be achieved. Furthermore, detection of TNF-α in mitogen stimulated whole blood was demonstrated.

Original languageEnglish (US)
Pages (from-to)4321-4326
Number of pages6
JournalAnalyst
Volume138
Issue number15
DOIs
StatePublished - Aug 7 2013
Externally publishedYes

Fingerprint

tumor
Blood
Tumor Necrosis Factor-alpha
blood
Point-of-Care Systems
Cytokines
Electrochemical sensors
Mitogens
Limit of Detection
sensor
Costs and Cost Analysis
Testing
cost
human TNF protein
detection
necrosis
Costs
analysis
marker

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

An aptasensor for electrochemical detection of tumor necrosis factor in human blood. / Liu, Ying; Zhou, Qing; Revzin, Alexander.

In: Analyst, Vol. 138, No. 15, 07.08.2013, p. 4321-4326.

Research output: Contribution to journalArticle

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