Abstract
Electrochemical aptasensors can detect disease markers such as cytokines to provide point-of-care diagnosis that is low-cost, rapid, specific and sensitive. Herein, we describe the development of an aptamer-based electrochemical sensor for detection and analysis of tumor necrosis factor-alpha (TNF-α)-a key inflammatory cytokine-in whole human blood. When testing spiked blood, a TNF-α detection limit of 58 pM (10 ng mL-1) and a linear range of 6 nM (100 ng mL-1) could be achieved. Furthermore, detection of TNF-α in mitogen stimulated whole blood was demonstrated.
Original language | English (US) |
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Pages (from-to) | 4321-4326 |
Number of pages | 6 |
Journal | Analyst |
Volume | 138 |
Issue number | 15 |
DOIs | |
State | Published - Aug 7 2013 |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy
- Electrochemistry