TY - GEN
T1 - Alveolar epithelial injury in vitro
T2 - Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
AU - Tschumperlin, D. J.
AU - Oswari, J.
AU - Margulies, S. S.
PY - 1999
Y1 - 1999
N2 - The onset of ventilator-induced lung injury (VILI) is linked to a number of possible mechanisms. To isolate the role alveolar epithelial deformation may play in the development of VILI, we have developed an in vitro system in which changes in alveolar epithelial cell viability can be measured after exposure to tightly controlled and physiologically relevant deformations. In this communication we explore the relative effect of deformation rate and duration on cell viability. Rat primary alveolar epithelial cells were exposed to a variety of cyclic biaxial stretch protocols, and deformation-induced injury was found to occur rapidly, with the majority of cell death occurring during the first five minutes of deformation. In addition, deformation-induced injury was found to be relatively insensitive to the rate of deformation.
AB - The onset of ventilator-induced lung injury (VILI) is linked to a number of possible mechanisms. To isolate the role alveolar epithelial deformation may play in the development of VILI, we have developed an in vitro system in which changes in alveolar epithelial cell viability can be measured after exposure to tightly controlled and physiologically relevant deformations. In this communication we explore the relative effect of deformation rate and duration on cell viability. Rat primary alveolar epithelial cells were exposed to a variety of cyclic biaxial stretch protocols, and deformation-induced injury was found to occur rapidly, with the majority of cell death occurring during the first five minutes of deformation. In addition, deformation-induced injury was found to be relatively insensitive to the rate of deformation.
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M3 - Conference contribution
AN - SCOPUS:0033313863
SN - 0780356756
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 32
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PB - IEEE
Y2 - 13 October 1999 through 16 October 1999
ER -