Alveolar epithelial injury in vitro: Temporal evolution and strain rate sensitivity

Daniel J Tschumperlin, J. Oswari, S. S. Margulies

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The onset of ventilator-induced lung injury (VILI) is linked to a number of possible mechanisms. To isolate the role alveolar epithelial deformation may play in the development of VILI, we have developed an in vitro system in which changes in alveolar epithelial cell viability can be measured after exposure to tightly controlled and physiologically relevant deformations. In this communication we explore the relative effect of deformation rate and duration on cell viability. Rat primary alveolar epithelial cells were exposed to a variety of cyclic biaxial stretch protocols, and deformation-induced injury was found to occur rapidly, with the majority of cell death occurring during the first five minutes of deformation. In addition, deformation-induced injury was found to be relatively insensitive to the rate of deformation.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Place of PublicationPiscataway, NJ, United States
PublisherIEEE
Pages32
Number of pages1
Volume1
ISBN (Print)0780356756
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

Fingerprint

Strain rate
Cell death
Rats
Cells
Communication

ASJC Scopus subject areas

  • Bioengineering

Cite this

Tschumperlin, D. J., Oswari, J., & Margulies, S. S. (1999). Alveolar epithelial injury in vitro: Temporal evolution and strain rate sensitivity. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 32). Piscataway, NJ, United States: IEEE.

Alveolar epithelial injury in vitro : Temporal evolution and strain rate sensitivity. / Tschumperlin, Daniel J; Oswari, J.; Margulies, S. S.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 Piscataway, NJ, United States : IEEE, 1999. p. 32.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tschumperlin, DJ, Oswari, J & Margulies, SS 1999, Alveolar epithelial injury in vitro: Temporal evolution and strain rate sensitivity. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 1, IEEE, Piscataway, NJ, United States, pp. 32, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.
Tschumperlin DJ, Oswari J, Margulies SS. Alveolar epithelial injury in vitro: Temporal evolution and strain rate sensitivity. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1. Piscataway, NJ, United States: IEEE. 1999. p. 32
Tschumperlin, Daniel J ; Oswari, J. ; Margulies, S. S. / Alveolar epithelial injury in vitro : Temporal evolution and strain rate sensitivity. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 Piscataway, NJ, United States : IEEE, 1999. pp. 32
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