Alveolar epithelial injury in vitro: Temporal evolution and strain rate sensitivity

D. J. Tschumperlin, J. Oswari, S. S. Margulies

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The onset of ventilator-induced lung injury (VILI) is linked to a number of possible mechanisms. To isolate the role alveolar epithelial deformation may play in the development of VILI, we have developed an in vitro system in which changes in alveolar epithelial cell viability can be measured after exposure to tightly controlled and physiologically relevant deformations. In this communication we explore the relative effect of deformation rate and duration on cell viability. Rat primary alveolar epithelial cells were exposed to a variety of cyclic biaxial stretch protocols, and deformation-induced injury was found to occur rapidly, with the majority of cell death occurring during the first five minutes of deformation. In addition, deformation-induced injury was found to be relatively insensitive to the rate of deformation.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Number of pages1
ISBN (Print)0780356756
StatePublished - Dec 1 1999
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
ISSN (Print)0589-1019

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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  • Cite this

    Tschumperlin, D. J., Oswari, J., & Margulies, S. S. (1999). Alveolar epithelial injury in vitro: Temporal evolution and strain rate sensitivity. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 1). IEEE.