Altered gene expression in human astrocytoma cells selected for migration: I. Thromboxane synthase

Wendy Mcdonough, Nhan Tran, Alf Giese, Sylvia A. Norman, Michael E. Berens

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Human glioma cells from a long-term cell line were selected for their ability to migrate on a glioma-derived extracellular matrix. When tested over 28 serial passages, the migration-selected strain showed a genetically stable, enhanced migration rate compared with the parental cells. Proliferation studies demonstrated that the growth rate of migration- selected cells was slightly arrested. Both the selected strain and the parental culture showed anchorage-independent growth in soft agarose and were tumorigenic in athymic mice. Using molecular genetic strategies' display to isolate genes expressed differentially between the 2 populations, a 300-bp sequence homologous to thromboxane synthase was upregulated in the migration- selected cells relative to the parental cells. Expression levels of thromboxane synthase were highly elevated in the migration-selected cells when assessed by RNAse-protection assay and by flow cytometry. Two specific thromboxane synthase inhibitors. Dazmegrel and Furegrelate, reduced the migration rate of the migration-selected cells to a rate equal to or less than the rate exhibited by the parental cells, respectively. The inhibitors effect on the parental cells was inconsequential. These results suggest that aberrations in the regulation of thromboxane synthase expression or activity may influence the motility of human glioma cells.

Original languageEnglish (US)
Pages (from-to)449-455
Number of pages7
JournalJournal of Neuropathology and Experimental Neurology
Volume57
Issue number5
DOIs
StatePublished - May 1998

Keywords

  • Dazmegrel
  • Differential display
  • Furegrelate
  • Glioma
  • Migration
  • Thromboxane synthase

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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