Alterations in carrier-mediated glutamine transport after a model of canine jejunal autotransplantation

Andrew J. Oishi, Yoshifumi Inoue, Wiley W. Souba, Michael G. Sarr

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The effects of small bowel transplantation (SBTx) on absorptive function are unknown. Preliminary experiments showed a decrease in absorption of glutamine. Our aim was to determine mechanisms of decreased ileal transport of glutamine utilizing a model of intestinal autotransplantation. Seven dogs were studied before and after a model of jejunoileal autotransplantation. In vivo absorption experiments were performed before and two and eight weeks postoperatively with an electrolyte solution containing glutamine (20 mM). In vitro glutamine transport was studied using brush-border membrane vesicles (BBMV) prepared from ileal mucosa obtained from six other dogs and compared to a controls. In vivo net absorptive flux of glutamine decreased at two weeks but returned toward baseline by eight weeks (P = 0.06). Transport of glutamine into BBMVs was decreased at two weeks and remained decreased at eight weeks. K(max)(Na+), a measure of carrier affinity was unchanged but V(max)(Na+), function of the number of transporter was decreased at two and eight weeks. Glucose transport was unchanged. It is concluded that jejunoileal autotransplantation decreases ileal absorption of glutamine by a decrease in carrier-mediated transport of glutamine.

Original languageEnglish (US)
Pages (from-to)1915-1924
Number of pages10
JournalDigestive Diseases and Sciences
Volume41
Issue number10
DOIs
StatePublished - 1996

Fingerprint

Autologous Transplantation
Glutamine
Canidae
Dogs
Microvilli
Electrolytes
Mucous Membrane
Transplantation
Glucose
Membranes

Keywords

  • Absorption
  • Amino acid absorption
  • Amino acid transport
  • Glutamine transport
  • Small bowel transplantation

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Alterations in carrier-mediated glutamine transport after a model of canine jejunal autotransplantation. / Oishi, Andrew J.; Inoue, Yoshifumi; Souba, Wiley W.; Sarr, Michael G.

In: Digestive Diseases and Sciences, Vol. 41, No. 10, 1996, p. 1915-1924.

Research output: Contribution to journalArticle

Oishi, Andrew J. ; Inoue, Yoshifumi ; Souba, Wiley W. ; Sarr, Michael G. / Alterations in carrier-mediated glutamine transport after a model of canine jejunal autotransplantation. In: Digestive Diseases and Sciences. 1996 ; Vol. 41, No. 10. pp. 1915-1924.
@article{619ee23234c04a4e9459c8f7296400be,
title = "Alterations in carrier-mediated glutamine transport after a model of canine jejunal autotransplantation",
abstract = "The effects of small bowel transplantation (SBTx) on absorptive function are unknown. Preliminary experiments showed a decrease in absorption of glutamine. Our aim was to determine mechanisms of decreased ileal transport of glutamine utilizing a model of intestinal autotransplantation. Seven dogs were studied before and after a model of jejunoileal autotransplantation. In vivo absorption experiments were performed before and two and eight weeks postoperatively with an electrolyte solution containing glutamine (20 mM). In vitro glutamine transport was studied using brush-border membrane vesicles (BBMV) prepared from ileal mucosa obtained from six other dogs and compared to a controls. In vivo net absorptive flux of glutamine decreased at two weeks but returned toward baseline by eight weeks (P = 0.06). Transport of glutamine into BBMVs was decreased at two weeks and remained decreased at eight weeks. K(max)(Na+), a measure of carrier affinity was unchanged but V(max)(Na+), function of the number of transporter was decreased at two and eight weeks. Glucose transport was unchanged. It is concluded that jejunoileal autotransplantation decreases ileal absorption of glutamine by a decrease in carrier-mediated transport of glutamine.",
keywords = "Absorption, Amino acid absorption, Amino acid transport, Glutamine transport, Small bowel transplantation",
author = "Oishi, {Andrew J.} and Yoshifumi Inoue and Souba, {Wiley W.} and Sarr, {Michael G.}",
year = "1996",
doi = "10.1007/BF02093590",
language = "English (US)",
volume = "41",
pages = "1915--1924",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Springer New York",
number = "10",

}

TY - JOUR

T1 - Alterations in carrier-mediated glutamine transport after a model of canine jejunal autotransplantation

AU - Oishi, Andrew J.

AU - Inoue, Yoshifumi

AU - Souba, Wiley W.

AU - Sarr, Michael G.

PY - 1996

Y1 - 1996

N2 - The effects of small bowel transplantation (SBTx) on absorptive function are unknown. Preliminary experiments showed a decrease in absorption of glutamine. Our aim was to determine mechanisms of decreased ileal transport of glutamine utilizing a model of intestinal autotransplantation. Seven dogs were studied before and after a model of jejunoileal autotransplantation. In vivo absorption experiments were performed before and two and eight weeks postoperatively with an electrolyte solution containing glutamine (20 mM). In vitro glutamine transport was studied using brush-border membrane vesicles (BBMV) prepared from ileal mucosa obtained from six other dogs and compared to a controls. In vivo net absorptive flux of glutamine decreased at two weeks but returned toward baseline by eight weeks (P = 0.06). Transport of glutamine into BBMVs was decreased at two weeks and remained decreased at eight weeks. K(max)(Na+), a measure of carrier affinity was unchanged but V(max)(Na+), function of the number of transporter was decreased at two and eight weeks. Glucose transport was unchanged. It is concluded that jejunoileal autotransplantation decreases ileal absorption of glutamine by a decrease in carrier-mediated transport of glutamine.

AB - The effects of small bowel transplantation (SBTx) on absorptive function are unknown. Preliminary experiments showed a decrease in absorption of glutamine. Our aim was to determine mechanisms of decreased ileal transport of glutamine utilizing a model of intestinal autotransplantation. Seven dogs were studied before and after a model of jejunoileal autotransplantation. In vivo absorption experiments were performed before and two and eight weeks postoperatively with an electrolyte solution containing glutamine (20 mM). In vitro glutamine transport was studied using brush-border membrane vesicles (BBMV) prepared from ileal mucosa obtained from six other dogs and compared to a controls. In vivo net absorptive flux of glutamine decreased at two weeks but returned toward baseline by eight weeks (P = 0.06). Transport of glutamine into BBMVs was decreased at two weeks and remained decreased at eight weeks. K(max)(Na+), a measure of carrier affinity was unchanged but V(max)(Na+), function of the number of transporter was decreased at two and eight weeks. Glucose transport was unchanged. It is concluded that jejunoileal autotransplantation decreases ileal absorption of glutamine by a decrease in carrier-mediated transport of glutamine.

KW - Absorption

KW - Amino acid absorption

KW - Amino acid transport

KW - Glutamine transport

KW - Small bowel transplantation

UR - http://www.scopus.com/inward/record.url?scp=0029977957&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029977957&partnerID=8YFLogxK

U2 - 10.1007/BF02093590

DO - 10.1007/BF02093590

M3 - Article

C2 - 8888701

AN - SCOPUS:0029977957

VL - 41

SP - 1915

EP - 1924

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 10

ER -