Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells

Elizabeth Jacobsen, Sergei I. Ochkur, Ralph S. Pero, Anna G. Taranova, Cheryl A. Protheroe, Dana C. Colbert, Nancy A Lee, James J. Lee

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

The current paradigm surrounding allergen-mediated T helper type 2 (Th2) immune responses in the lung suggests an almost hegemonic role for T cells. Our studies propose an alternative hypothesis implicating eosinophils in the regulation of pulmonary T cell responses. In particular, ovalbumin (OVA)-sensitized/challenged mice devoid of eosinophils (the transgenic line PHIL) have reduced airway levels of Th2 cytokines relative to the OVA-treated wild type that correlated with a reduced ability to recruit effector T cells to the lung. Adoptive transfer of Th2-polarized OVA-specific transgenic T cells (OT-II) alone into OVA-challenged PHIL recipient mice failed to restore Th2 cytokines, airway histopathologies, and, most importantly, the recruitment of pulmonary effector T cells. In contrast, the combined transfer of OT-II cells and eosinophils into PHIL mice resulted in the accumulation of effector T cells and a concomitant increase in both airway Th2 immune responses and histopathologies. Moreover, we show that eosinophils elicit the expression of the Th2 chemokines thymus- and activation-regulated chemokine/CCL17 and macrophage-derived chemokine/CCL22 in the lung after allergen challenge, and blockade of these chemokines inhibited the recruitment of effector T cells. In summary, the data suggest that pulmonary eosinophils are required for the localized recruitment of effector T cells. JEM

Original languageEnglish (US)
Pages (from-to)699-710
Number of pages12
JournalJournal of Experimental Medicine
Volume205
Issue number3
DOIs
StatePublished - Mar 17 2008

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Eosinophils
Pneumonia
T-Lymphocytes
Ovalbumin
Lung
Chemokine CCL22
Chemokine CCL17
Chemokines
Allergens
Cytokines
Adoptive Transfer

ASJC Scopus subject areas

  • Immunology

Cite this

Jacobsen, E., Ochkur, S. I., Pero, R. S., Taranova, A. G., Protheroe, C. A., Colbert, D. C., ... Lee, J. J. (2008). Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells. Journal of Experimental Medicine, 205(3), 699-710. https://doi.org/10.1084/jem.20071840

Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells. / Jacobsen, Elizabeth; Ochkur, Sergei I.; Pero, Ralph S.; Taranova, Anna G.; Protheroe, Cheryl A.; Colbert, Dana C.; Lee, Nancy A; Lee, James J.

In: Journal of Experimental Medicine, Vol. 205, No. 3, 17.03.2008, p. 699-710.

Research output: Contribution to journalArticle

Jacobsen, Elizabeth ; Ochkur, Sergei I. ; Pero, Ralph S. ; Taranova, Anna G. ; Protheroe, Cheryl A. ; Colbert, Dana C. ; Lee, Nancy A ; Lee, James J. / Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells. In: Journal of Experimental Medicine. 2008 ; Vol. 205, No. 3. pp. 699-710.
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