TY - JOUR
T1 - Allelic diversity at minisatellite MS205 (D16S309)
T2 - Evidence for polarized variability
AU - Armour, John A.L.
AU - Harris, Peter C.
AU - Jeffreys, Alec J.
N1 - Funding Information:
We thank Dr Raymond Stallings (Los Alamos National Laboratory, Los Alamos, NM) for cosmid c302G6; Nicola Royle, Darren Monckton, David Neil, Keiji Tamaki and an anonymous reviewer for helpful comments; Profs. Howard Cann and Jean Dausset at CEPH for DNA samples; and Debra Langton for oligonucleotide synthesis. JALA is a Wellcome Trust Postdoctoral Fellow. The research of AJJ was supported in part by an International Research Scholars award from the Howard Hughes Medical Institute. This work was also supported by grants to AJJ from the MRC and the Royal Society.
PY - 1993/8
Y1 - 1993/8
N2 - We have determined the allelic structures on 106 Caucasian chromosomes at the minisatellite locus MS205 (D16S309). In addition to the Internal structures deduced by minisatellite variant repeat (MVR) mapping, the genotypes at six flanking substitutional polymorphic sites have been analysed. The minisatellite structures show a polarity of variation at MS205, with most observed variation due to differences at one extremity of the tandem repeat array. This locus, therefore, provides a further example of polarity of variation at human minisatellites. Analysis of haplotypes at flanking polymorphic sites suggests that there may be a higher frequency of exchange near the highly unstable end of the minisatellite, and thus that exchanges of flanking polymorphisms may be due to co-conversion or recombination events occurring during unequal exchanges between minisatellite alleles.
AB - We have determined the allelic structures on 106 Caucasian chromosomes at the minisatellite locus MS205 (D16S309). In addition to the Internal structures deduced by minisatellite variant repeat (MVR) mapping, the genotypes at six flanking substitutional polymorphic sites have been analysed. The minisatellite structures show a polarity of variation at MS205, with most observed variation due to differences at one extremity of the tandem repeat array. This locus, therefore, provides a further example of polarity of variation at human minisatellites. Analysis of haplotypes at flanking polymorphic sites suggests that there may be a higher frequency of exchange near the highly unstable end of the minisatellite, and thus that exchanges of flanking polymorphisms may be due to co-conversion or recombination events occurring during unequal exchanges between minisatellite alleles.
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U2 - 10.1093/hmg/2.8.1137
DO - 10.1093/hmg/2.8.1137
M3 - Article
C2 - 8401495
AN - SCOPUS:0027209516
SN - 0964-6906
VL - 2
SP - 1137
EP - 1145
JO - Human molecular genetics
JF - Human molecular genetics
IS - 8
ER -