Abstract
The mechanisms underlying effects of aging on functions of pro-angiogenic endothelial progenitor cells (EPCs) are poorly understood. Previous studies demonstrated that human EPCs express high levels of antioxidant enzymes as compared to mature endothelial cells. Here, we hypothesized that aging impairs antioxidant capacity of EPCs. So called "early EPCs" derived from cultured blood mononuclear cells were obtained from healthy young (average = 24 years old) and old (average = 72 years old) subjects. In EPCs of old subjects, the levels of glutathione peroxidase-1 (GPX1) protein and enzymatic activity were significantly reduced. The serum selenium levels in young and old subjects were not significantly different. Increasing selenium concentration in the cell culture also did not affect the protein levels of GPX1, suggesting the reduced GPX1 in old subject's EPCs is selenium independent. Expressions of catalase, Mn-superoxide dismutase (MnSOD), and CuZnSOD were not affected by aging. EPCs of old subjects were more sensitive to oxidative stress induced by H2O2 as compared with EPCs of young subjects, suggesting that impairment of GPX1 during aging may contribute to low survival ability of EPCs in response to oxidative stress. The results indicate that GPX1 may represent a potential therapeutic target for enhancement of regenerative capacity of EPCs in old subjects.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 447-452 |
| Number of pages | 6 |
| Journal | Microvascular Research |
| Volume | 78 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 2009 |
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Keywords
- Aging
- Endothelial progenitor cells
- GPX1
- Human subjects
- Oxidative stress
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Cardiology and Cardiovascular Medicine
Cite this
Aging decreases expression and activity of glutathione peroxidase-1 in human endothelial progenitor cells. / He, Tongrong; Joyner, Michael Joseph; Katusic, Zvonimir S.
In: Microvascular Research, Vol. 78, No. 3, 12.2009, p. 447-452.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aging decreases expression and activity of glutathione peroxidase-1 in human endothelial progenitor cells
AU - He, Tongrong
AU - Joyner, Michael Joseph
AU - Katusic, Zvonimir S
PY - 2009/12
Y1 - 2009/12
N2 - The mechanisms underlying effects of aging on functions of pro-angiogenic endothelial progenitor cells (EPCs) are poorly understood. Previous studies demonstrated that human EPCs express high levels of antioxidant enzymes as compared to mature endothelial cells. Here, we hypothesized that aging impairs antioxidant capacity of EPCs. So called "early EPCs" derived from cultured blood mononuclear cells were obtained from healthy young (average = 24 years old) and old (average = 72 years old) subjects. In EPCs of old subjects, the levels of glutathione peroxidase-1 (GPX1) protein and enzymatic activity were significantly reduced. The serum selenium levels in young and old subjects were not significantly different. Increasing selenium concentration in the cell culture also did not affect the protein levels of GPX1, suggesting the reduced GPX1 in old subject's EPCs is selenium independent. Expressions of catalase, Mn-superoxide dismutase (MnSOD), and CuZnSOD were not affected by aging. EPCs of old subjects were more sensitive to oxidative stress induced by H2O2 as compared with EPCs of young subjects, suggesting that impairment of GPX1 during aging may contribute to low survival ability of EPCs in response to oxidative stress. The results indicate that GPX1 may represent a potential therapeutic target for enhancement of regenerative capacity of EPCs in old subjects.
AB - The mechanisms underlying effects of aging on functions of pro-angiogenic endothelial progenitor cells (EPCs) are poorly understood. Previous studies demonstrated that human EPCs express high levels of antioxidant enzymes as compared to mature endothelial cells. Here, we hypothesized that aging impairs antioxidant capacity of EPCs. So called "early EPCs" derived from cultured blood mononuclear cells were obtained from healthy young (average = 24 years old) and old (average = 72 years old) subjects. In EPCs of old subjects, the levels of glutathione peroxidase-1 (GPX1) protein and enzymatic activity were significantly reduced. The serum selenium levels in young and old subjects were not significantly different. Increasing selenium concentration in the cell culture also did not affect the protein levels of GPX1, suggesting the reduced GPX1 in old subject's EPCs is selenium independent. Expressions of catalase, Mn-superoxide dismutase (MnSOD), and CuZnSOD were not affected by aging. EPCs of old subjects were more sensitive to oxidative stress induced by H2O2 as compared with EPCs of young subjects, suggesting that impairment of GPX1 during aging may contribute to low survival ability of EPCs in response to oxidative stress. The results indicate that GPX1 may represent a potential therapeutic target for enhancement of regenerative capacity of EPCs in old subjects.
KW - Aging
KW - Endothelial progenitor cells
KW - GPX1
KW - Human subjects
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=71549137764&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=71549137764&partnerID=8YFLogxK
U2 - 10.1016/j.mvr.2009.08.009
DO - 10.1016/j.mvr.2009.08.009
M3 - Article
C2 - 19733578
AN - SCOPUS:71549137764
VL - 78
SP - 447
EP - 452
JO - Microvascular Research
JF - Microvascular Research
SN - 0026-2862
IS - 3
ER -