Affinity thresholds for membrane fusion triggering by viral glycoproteins

Kosei Hasegawa, Chunling Hu, Takafumi Nakamura, James D. Marks, Stephen J Russell, Kah-Whye Peng

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Enveloped viruses trigger membrane fusion to gain entry into cells. The receptor affinities of their attachment proteins vary greatly, from 10 -4 M to 10-9 M, but the significance of this is unknown. Using six retargeted measles viruses that bind to Her-2/neu with a 5-log range in affinity, we show that receptor affinity has little impact on viral attachment but is nevertheless a key determinant of infectivity and intercellular fusion. For a given cell surface receptor density, there is an affinity threshold above which cell-cell fusion proceeds efficiently. Suprathreshold affinities do not further enhance the efficiency of membrane fusion.

Original languageEnglish (US)
Pages (from-to)13149-13157
Number of pages9
JournalJournal of Virology
Volume81
Issue number23
DOIs
StatePublished - Dec 2007

Fingerprint

Viral Fusion Proteins
Membrane Fusion
glycoproteins
Virus Internalization
Measles virus
receptors
Cell Fusion
Cell Surface Receptors
cell fusion
cells
pathogenicity
viruses
Proteins
membrane fusion
proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Affinity thresholds for membrane fusion triggering by viral glycoproteins. / Hasegawa, Kosei; Hu, Chunling; Nakamura, Takafumi; Marks, James D.; Russell, Stephen J; Peng, Kah-Whye.

In: Journal of Virology, Vol. 81, No. 23, 12.2007, p. 13149-13157.

Research output: Contribution to journalArticle

Hasegawa, Kosei ; Hu, Chunling ; Nakamura, Takafumi ; Marks, James D. ; Russell, Stephen J ; Peng, Kah-Whye. / Affinity thresholds for membrane fusion triggering by viral glycoproteins. In: Journal of Virology. 2007 ; Vol. 81, No. 23. pp. 13149-13157.
@article{cebd31ca445c4b11bfa66071e3155782,
title = "Affinity thresholds for membrane fusion triggering by viral glycoproteins",
abstract = "Enveloped viruses trigger membrane fusion to gain entry into cells. The receptor affinities of their attachment proteins vary greatly, from 10 -4 M to 10-9 M, but the significance of this is unknown. Using six retargeted measles viruses that bind to Her-2/neu with a 5-log range in affinity, we show that receptor affinity has little impact on viral attachment but is nevertheless a key determinant of infectivity and intercellular fusion. For a given cell surface receptor density, there is an affinity threshold above which cell-cell fusion proceeds efficiently. Suprathreshold affinities do not further enhance the efficiency of membrane fusion.",
author = "Kosei Hasegawa and Chunling Hu and Takafumi Nakamura and Marks, {James D.} and Russell, {Stephen J} and Kah-Whye Peng",
year = "2007",
month = "12",
doi = "10.1128/JVI.01415-07",
language = "English (US)",
volume = "81",
pages = "13149--13157",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "23",

}

TY - JOUR

T1 - Affinity thresholds for membrane fusion triggering by viral glycoproteins

AU - Hasegawa, Kosei

AU - Hu, Chunling

AU - Nakamura, Takafumi

AU - Marks, James D.

AU - Russell, Stephen J

AU - Peng, Kah-Whye

PY - 2007/12

Y1 - 2007/12

N2 - Enveloped viruses trigger membrane fusion to gain entry into cells. The receptor affinities of their attachment proteins vary greatly, from 10 -4 M to 10-9 M, but the significance of this is unknown. Using six retargeted measles viruses that bind to Her-2/neu with a 5-log range in affinity, we show that receptor affinity has little impact on viral attachment but is nevertheless a key determinant of infectivity and intercellular fusion. For a given cell surface receptor density, there is an affinity threshold above which cell-cell fusion proceeds efficiently. Suprathreshold affinities do not further enhance the efficiency of membrane fusion.

AB - Enveloped viruses trigger membrane fusion to gain entry into cells. The receptor affinities of their attachment proteins vary greatly, from 10 -4 M to 10-9 M, but the significance of this is unknown. Using six retargeted measles viruses that bind to Her-2/neu with a 5-log range in affinity, we show that receptor affinity has little impact on viral attachment but is nevertheless a key determinant of infectivity and intercellular fusion. For a given cell surface receptor density, there is an affinity threshold above which cell-cell fusion proceeds efficiently. Suprathreshold affinities do not further enhance the efficiency of membrane fusion.

UR - http://www.scopus.com/inward/record.url?scp=36348991497&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36348991497&partnerID=8YFLogxK

U2 - 10.1128/JVI.01415-07

DO - 10.1128/JVI.01415-07

M3 - Article

C2 - 17804513

AN - SCOPUS:36348991497

VL - 81

SP - 13149

EP - 13157

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 23

ER -