Adipose tissue DNA methylome changes in development of new-onset diabetes after kidney transplantation

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2 Citations (Scopus)

Abstract

Aim: New-onset diabetes after kidney transplant (NODAT) adversely impacts kidney allograft and patient survival. Epigenetic alterations in adipose tissue like DNA methylation may play a contributory role. Methods: Adipose tissue DNA of the patients with NODAT and their age, sex and BMI matched controls (nine each) were sequenced by reduced representation bisulfite sequencing. Differentially methylated CpGs (DMCs) and differentially methylated regions (DMRs) were studied. Results: Adipose tissue from the patients had reduced DNA methylation in intergenic and intronic regions. DMCs were found to be more hypomethylated in repeat regions and hypermethylated in CGIs and promoter region. About 900 DMRs were found and their associated genes were significantly enriched in 32 pathways, the top ones of which were associated with insulin resistance and inflammation. Some DMR or DMC genes have known T2DM associations. Conclusion: Changes in DNA methylation in adipose tissue may be suggestive of future NODAT.

Original languageEnglish (US)
Pages (from-to)1423-1435
Number of pages13
JournalEpigenomics
Volume9
Issue number11
DOIs
StatePublished - Nov 1 2017

Fingerprint

Kidney Transplantation
Adipose Tissue
DNA Methylation
Kidney
DNA
Transplants
Intergenic DNA
Genetic Promoter Regions
Epigenomics
Genes
Allografts
Insulin Resistance
Inflammation
Survival

Keywords

  • adipose tissue
  • DNA methylation
  • new-onset diabetes after transplant
  • RRBS

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

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title = "Adipose tissue DNA methylome changes in development of new-onset diabetes after kidney transplantation",
abstract = "Aim: New-onset diabetes after kidney transplant (NODAT) adversely impacts kidney allograft and patient survival. Epigenetic alterations in adipose tissue like DNA methylation may play a contributory role. Methods: Adipose tissue DNA of the patients with NODAT and their age, sex and BMI matched controls (nine each) were sequenced by reduced representation bisulfite sequencing. Differentially methylated CpGs (DMCs) and differentially methylated regions (DMRs) were studied. Results: Adipose tissue from the patients had reduced DNA methylation in intergenic and intronic regions. DMCs were found to be more hypomethylated in repeat regions and hypermethylated in CGIs and promoter region. About 900 DMRs were found and their associated genes were significantly enriched in 32 pathways, the top ones of which were associated with insulin resistance and inflammation. Some DMR or DMC genes have known T2DM associations. Conclusion: Changes in DNA methylation in adipose tissue may be suggestive of future NODAT.",
keywords = "adipose tissue, DNA methylation, new-onset diabetes after transplant, RRBS",
author = "Saurabh Baheti and Prachi Singh and Yun Zhang and Jared Evans and Jensen, {Michael Dennis} and Virend Somers and Jean-Pierre Kocher and Sun, {Zhifu D} and Chakkera, {Harini M}",
year = "2017",
month = "11",
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doi = "10.2217/epi-2017-0050",
language = "English (US)",
volume = "9",
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journal = "Epigenomics",
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publisher = "Future Medicine Ltd.",
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TY - JOUR

T1 - Adipose tissue DNA methylome changes in development of new-onset diabetes after kidney transplantation

AU - Baheti, Saurabh

AU - Singh, Prachi

AU - Zhang, Yun

AU - Evans, Jared

AU - Jensen, Michael Dennis

AU - Somers, Virend

AU - Kocher, Jean-Pierre

AU - Sun, Zhifu D

AU - Chakkera, Harini M

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Aim: New-onset diabetes after kidney transplant (NODAT) adversely impacts kidney allograft and patient survival. Epigenetic alterations in adipose tissue like DNA methylation may play a contributory role. Methods: Adipose tissue DNA of the patients with NODAT and their age, sex and BMI matched controls (nine each) were sequenced by reduced representation bisulfite sequencing. Differentially methylated CpGs (DMCs) and differentially methylated regions (DMRs) were studied. Results: Adipose tissue from the patients had reduced DNA methylation in intergenic and intronic regions. DMCs were found to be more hypomethylated in repeat regions and hypermethylated in CGIs and promoter region. About 900 DMRs were found and their associated genes were significantly enriched in 32 pathways, the top ones of which were associated with insulin resistance and inflammation. Some DMR or DMC genes have known T2DM associations. Conclusion: Changes in DNA methylation in adipose tissue may be suggestive of future NODAT.

AB - Aim: New-onset diabetes after kidney transplant (NODAT) adversely impacts kidney allograft and patient survival. Epigenetic alterations in adipose tissue like DNA methylation may play a contributory role. Methods: Adipose tissue DNA of the patients with NODAT and their age, sex and BMI matched controls (nine each) were sequenced by reduced representation bisulfite sequencing. Differentially methylated CpGs (DMCs) and differentially methylated regions (DMRs) were studied. Results: Adipose tissue from the patients had reduced DNA methylation in intergenic and intronic regions. DMCs were found to be more hypomethylated in repeat regions and hypermethylated in CGIs and promoter region. About 900 DMRs were found and their associated genes were significantly enriched in 32 pathways, the top ones of which were associated with insulin resistance and inflammation. Some DMR or DMC genes have known T2DM associations. Conclusion: Changes in DNA methylation in adipose tissue may be suggestive of future NODAT.

KW - adipose tissue

KW - DNA methylation

KW - new-onset diabetes after transplant

KW - RRBS

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