Adhesion of uric acid crystals to the surface of renal epithelial cells

Rima M. Koka, Erick Huang, John C Lieske

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Adhesion of microcrystals that nucleate in tubular fluid to the apical surface of renal tubular cells could be a critical step in the formation of kidney stones, 12% of which contain uric acid (UA) either alone or admixed with calcium oxalates or calcium phosphates. UA crystals bind rapidly to monolayer cultures of monkey kidney epithelial cells (BSC-1 line), used to model the surface of the nephron, in a concentration-dependent manner. The urinary glycoproteins osteopontin, nephrocalcin, and Tamm-Horsfall glycoprotein had no effect on binding of UA crystals to the cell surface, whereas other polyanions including specific glycosaminoglycans blocked UA crystal adhesion. Specific polycations also inhibited adhesion of UA crystals and appeared to exert their inhibitory effect by coating cells. However, removal of anionic cell surface molecules with neuraminidase, heparitinase I, or chondroitinase ABC each increased UA crystal binding, and sialic acid- binding lectins had no effect. These observations suggest that hydrogen bonding and hydrophobic interactions play a major role in adhesion of electrostatically neutral UA crystals to renal cells, unlike the interaction of calcium-containing crystals with negatively charged molecules on the apical cell surface via ionic forces. After adhesion to the plasma membrane, subsequent cellular events could contribute to UA crystal retention in the kidney and the development of UA or mixed calcium and UA calculi.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume278
Issue number6 47-6
StatePublished - Jun 2000

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Uric Acid
Epithelial Cells
Kidney
heparitinsulfate lyase
Calcium Phosphates
Uromodulin
Chondroitin ABC Lyase
Calcium
Calcium Oxalate
Osteopontin
Kidney Calculi
Nephrons
Calculi
Neuraminidase
N-Acetylneuraminic Acid
Hydrogen Bonding
Glycosaminoglycans
Hydrophobic and Hydrophilic Interactions
Lectins
Cell Communication

Keywords

  • 3T3 fibroblasts
  • BSC-1 cells
  • Glycosaminoglycans
  • Madin-Darby canine kidney cells
  • Nephrolithiasis

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Adhesion of uric acid crystals to the surface of renal epithelial cells. / Koka, Rima M.; Huang, Erick; Lieske, John C.

In: American Journal of Physiology - Renal Physiology, Vol. 278, No. 6 47-6, 06.2000.

Research output: Contribution to journalArticle

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