Acute impairment of endothelium-dependent relaxations to aggregating platelets following reperfusion injury in canine coronary arteries

P. J. Pearson, Hartzell V Schaff, P. M. Vanhoutte

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Experiments were designed to determine whether endothelial injury contributes to augmented coronary vascular tone seen during myocardial reperfusion. Canine left anterior descending coronary arteries were exposed to ischemia followed by reperfusion (60 minutes each). Rings (3-4 mm) of the reperfused artery and of normal left circumflex (control) coronary artery segments were prepared. Rings were suspended for isometric force measurement in organ chamber containing modified Krebs' Ringer bicarbonate solution (37°C, 95%, O2-5% CO2). Endothelium-independent contractions to KCl and prostaglandin F(2α) were unaltered after reperfusion. Endothelium-dependent relaxations to nitric oxide, sodium nitroprusside, and isoproterenol were comparable in control and reperfused arteries. However, reperfused coronary arteries contracted with prostaglandin F(2α) lost the ability to express endothelium-dependent relaxations to aggregating platelets. Reperfused arterial rings also exhibited impaired endothelium-dependent relaxations to acetylcholine, the calcium ionophore A23187, and the platelet-derived compounds ADP and serotonin. Quiescent (noncontracted) reperfused arterial rings exhibited larger conractions than controls when exposed to aggregating platelets. In such quiescent rings, the endothelium-dependent increase in tension to hemoglobin was unaltered after reperfusion. Thus, coronary reperfusion impairs the normal endothelium-dependent relaxations to aggregating platelets and vasoactive drugs. This impairment of platelet-mediated coronary relaxtion could help explain the increased vascular tone and tendency toward vasospasm commonly observed after reperfusion of the coronary arteries.

Original languageEnglish (US)
Pages (from-to)385-393
Number of pages9
JournalCirculation Research
Volume67
Issue number2
StatePublished - 1990
Externally publishedYes

Fingerprint

Reperfusion Injury
Endothelium
Canidae
Coronary Vessels
Blood Platelets
Reperfusion
Myocardial Reperfusion
Prostaglandins F
Blood Vessels
Arteries
Calcium Ionophores
Calcimycin
Nitroprusside
Bicarbonates
Isoproterenol
Adenosine Diphosphate
Acetylcholine
Serotonin
Nitric Oxide
Hemoglobins

Keywords

  • ADP
  • Ischemia
  • Serotonin
  • Thrombosis
  • Vasospasm

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Acute impairment of endothelium-dependent relaxations to aggregating platelets following reperfusion injury in canine coronary arteries. / Pearson, P. J.; Schaff, Hartzell V; Vanhoutte, P. M.

In: Circulation Research, Vol. 67, No. 2, 1990, p. 385-393.

Research output: Contribution to journalArticle

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