Activation of a cell-cycle-regulated histone gene by the oncogenic transcription factor IRF-2

Patricia S. Vaughan, Farah Aziz, André J. van Wijnen, Shujian Wu, Hisashi Harada, Tadatsugu Taniguchi, Kenneth J. Soprano, Janet L. Stein, Gary S. Stein

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Abstract

THE human histone H4 gene FO108 is regulated during the cell cycle with a peak in transcription during early S phase1, 2. The cell-cycle element (CCE) required for H4 histone activation is a sequence of 11 base pairs that binds a protein factor in electrophor-etic mobility shift assays that has been designated histone nuclear factor M (HiNF-M)2, 3. Here we report the purification of HiNF-M, and show it to be a protein of relative molecular mass (Mr) 48K that is identical to interferon (IFN) regulatory factor 2 (IRF-2), a negative transcriptional regulator of the IFN response4. Recombinant IRF-2 (as well as the related protein IRF-1 (ref. 5)) binds the CCE specifically and activates transcription of this H4 histone gene. IRF-2 has been shown to have oncogenic potential6, and our results demonstrate a link between IRF-2 and a gene that is functionally coupled to DNA replication and cell-cycle progression at the Gl/S phase transition.

Original languageEnglish (US)
Pages (from-to)362-365
Number of pages4
JournalNature
Volume377
Issue number6547
DOIs
StatePublished - Sep 28 1995

ASJC Scopus subject areas

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    Vaughan, P. S., Aziz, F., van Wijnen, A. J., Wu, S., Harada, H., Taniguchi, T., Soprano, K. J., Stein, J. L., & Stein, G. S. (1995). Activation of a cell-cycle-regulated histone gene by the oncogenic transcription factor IRF-2. Nature, 377(6547), 362-365. https://doi.org/10.1038/377362a0