AcMNPV ORF38 protein has the activity of ADP-ribose pyrophosphatase and is important for virus replication

Jing Ge, Zong Wei, Yishu Huang, Juan Yin, Ziqian Zhou, Jiang Zhong

Research output: Contribution to journalArticlepeer-review

Abstract

The ORF38 of Autographa californica multiple nucleopolyhedrovirus (AcMNPV), or AcORF38, contains a conserved motif of Nudix (nucleotide diphosphate X) superfamily. It has the highest homology with ADP-ribose pyrophosphatase (ADPRase), a subfamily of Nudix pyrophosphatase. In the current study, recombinant AcORF38 protein was prepared and shown to have ADPRase activity, with a Km of 204 μM, and Kcat of 6.96 s- 1 at pH 8.0 and 5 mM MgCl2. The transcription of AcORF38 was detected 2 h postinfection, and lasted until the late stage. An orf38 gene-deleted mutant virus, vAcGFP-Δ38, was constructed. Although it produced progeny virus, the yield of extracellular virus was less than 1% of the wild-type virus. The activity of viral very late gene promoter was also greatly reduced in vAcGFP-Δ38-infected cells as indicated by the expression of green fluorescence protein gene driven by polyhedrin promoter. The mutant phenotype was rescued by co-transfection with an AcORF38-expressing plasmid. These results suggest that AcORF38 plays an important role in virus replication, although the detail is to be elucidated.

Original languageEnglish (US)
Pages (from-to)204-211
Number of pages8
JournalVirology
Volume361
Issue number1
DOIs
StatePublished - Apr 25 2007

Keywords

  • ADP-ribose pyrophosphatase
  • AcMNPV
  • Baculovirus
  • Nudix pyrophosphatase
  • ORF38
  • Virus replication

ASJC Scopus subject areas

  • Virology

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